Directed evolution of glutathione transferases towards a selective glutathione-binding site and improved oxidative stability - Axarli, Irine; Muleta, Abdi W.; Chronopoulou, Evangelia G.; Papageorgiou, Anastassios C.; Labrou, Nikolaos E. (Biochimica et biophysica acta. General subjects, 2017)
Keywords: adsorbents; Adsorption; affinity chromatography; Amino Acid Sequence; Animals; Binding Sites; biosensors; biotechnology; chimerism; crystal structure; Crystallography, X-Ray; Directed evolution; DNA; DNA Shuffling; DNA, Complementary - genetics; Enzyme Activation; Enzyme Stability; glutathione; Glutathione - metabolism; glutathione transferase; Glutathione Transferase - chemistry; Glutathione Transferase - metabolism; Glutathione transferase A1-1; Humans; Isoenzymes - chemistry; Isoenzymes - metabolism; Kinetics; Models, Molecular; mutagenesis; Mutagenesis, Site-Directed; Oxidation-Reduction; oxidative stability; point mutation; Protein stability; Rats; screening; Temperature; thermal stability; X-ray diffraction; X-ray structure; hGSTA1-1; Directed evolution; Glutathione transferase A1-1; GST; Protein stability; CDNB; rGSTA1-1; Sj26GST; GSH; X-ray structure