Detection and characterisation of radicals in biological materials using EPR methodology

• Published in: Biochimica et biophysica acta Vol. 1840; no. 2; pp. 708 - 721
• Main Authors: Hawkins, Clare L., Davies, Michael J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2014
• Subjects: Animals; biophysics; copper; Electron paramagnetic resonance; electron paramagnetic resonance spectroscopy; Electron Spin Resonance Spectroscopy - methods; Free Radicals - analysis; Humans; membrane proteins; metal ions; Nitric oxide; Nitroxide; Radical; reactive oxygen species; Spin trapping; Spin Trapping - methods; Superoxide; temperature; NO; O2; DEPMPO; EPR; EMPO; Nitroxide; Spin trapping; Radical; MNP; CYPMPO; BMPO; Hb; PBN; DTCS; ESR; MS; DMPO; SOD; Superoxide; MGD; POBN; DETC; Nitric oxide; Electron paramagnetic resonance; DBNBS; 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide; N-methyl-d-glucamine; 5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; electron spin resonance; haemoglobin; α-(4-pyridyl-1-oxide)-N-tert-butylnitrone; N-(dithiocarboxy)sarcosine; mass spectrometry; 5-(ethoxycarbonyl)-5-methyl-1-pyrroline N-oxide; 3,5-dibromo-4-nitrosobenzene sulfonic acid; N-tert-butyl-α-phenylnitrone; 5-(2,2-dimethyl-1,3-propoxycyclophosphoryl)-5-methyl-1-pyrroline N-oxide; 5,5-dimethyl-1-pyrroline N-oxide; superoxide radical anion; superoxide dismutase; O(−); diethyldithiocarbamate; 2-methyl-2-nitrosopropane

Electron paramagnetic resonance (EPR) spectroscopy (also known as electron spin resonance, ESR, spectroscopy) is widely considered to be the “gold standard” for the detection and characterisation of radicals in biological systems. The article reviews the major positive and negative aspects of EPR sp...