Characterizing the mode of action of extracellular Connexin43 channel blocking mimetic peptides in an in vitro ischemia injury model

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 2; pp. 68 - 78
• Main Authors: Kim, Yeri, Griffin, Jarred M., Harris, Paul W.R., Chan, Sin Hang Crystal, Nicholson, Louise F.B., Brimble, Margaret A., O'Carroll, Simon J., Green, Colin R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: adenosine triphosphate; Adenosine Triphosphate - metabolism; amino acid substitution; Cell Line; central nervous system; Central Nervous System Diseases - drug therapy; Central Nervous System Diseases - metabolism; Cerebrum - drug effects; Cerebrum - metabolism; chronic diseases; Connexin 43 - metabolism; Connexin43; endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Gap junctions; Gap Junctions - drug effects; Gap Junctions - metabolism; Hemichannel; Humans; Ion Channels - metabolism; Ischemia; Ischemia - drug therapy; Ischemia - metabolism; mechanism of action; Peptide5; peptides; Peptides - pharmacology; Peptidomimetics; permeability; Reperfusion Injury - drug therapy; Reperfusion Injury - metabolism; Retinal Diseases - drug therapy; Retinal Diseases - metabolism; Peptide5; Ischemia; Peptidomimetics; Gap junctions; CNS; Connexin43; Hemichannel; ATP; hCMVEC
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2016.11.001

Non-selective Connexin43 hemichannels contribute to secondary lesion spread. The hemichannel blocking peptidomimetic Peptide5, derived from the second extracellular loop of the human Connexin43 protein, prevents lesion spread and reduces vascular permeability in preclinical models of central nervous...