An in vitro investigation of the inhibitory mechanism of β-galactosidase by cinnamaldehyde alone and in combination with carvacrol and thymol

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 1; pp. 3189 - 3198
• Main Authors: Wang, Lang-Hong, Wang, Man-Sheng, Zeng, Xin-An, Gong, De-Ming, Huang, Yan-Bo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2017
• Subjects: Acrolein - analogs & derivatives; Acrolein - chemistry; Acrolein - pharmacology; active sites; antibiotics; atomic force microscopy; bark; beta-galactosidase; beta-Galactosidase - antagonists & inhibitors; beta-Galactosidase - metabolism; Binding Sites; bioactive properties; biochemical pathways; carvacrol; Cinnamaldehyde; cinnamon; Circular Dichroism; circular dichroism spectroscopy; Cluster Analysis; Competitive inhibition; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; fluorescence; Hydrogen-Ion Concentration; ingredients; Kinetics; leaves; mechanism of action; Microscopy, Atomic Force; Molecular Docking Simulation; Molecular modeling; molecular models; Monoterpenes - chemistry; Monoterpenes - pharmacology; nucleic acids; proteins; Spectrometry, Fluorescence; synergism; Synergistic effect; thymol; Thymol - chemistry; Thymol - pharmacology; tryptophan; tyrosine; β-Galactosidase; Cinnamaldehyde; Synergistic effect; Competitive inhibition; Molecular modeling; β-Galactosidase

Some antibacterial agents exert their antimicrobial action by targeting the cytoplasmic macromolecules, such as proteins or nucleic acids, to disturb the properties of macromolecules that may deeply influence their biological activities and functions. Cinnamaldehyde (CIN) is a natural antibacterial...