Single-molecule FRET reveals proofreading complexes in the large fragment of Bacillus stearothermophilus DNA polymerase I

• Published in: AIMS biophysics Vol. 5; no. 2; pp. 144 - 159
• Main Authors: Christian, Thomas V, Konigsberg, William H
• Format: Journal Article
• Language: English
• Published: United States AIMS Press 01.01.2018
• Subjects: base discrimination; pol-exo switching; proof-reading; smFRET; base discrimination; pol-exo switching; smFRET; proof-reading

There is increasing interest in the use of DNA polymerases (DNA pols) in next-generation sequencing strategies. These methodologies typically rely on members of the A and B family of DNA polymerases that are classified as high-fidelity DNA polymerases. These enzymes possess the ability to selectivel...