Antimicrobial peptide hybrid fluorescent protein based sensor array discriminate ten most frequent clinic isolates

• Published in: Biochimica et biophysica acta. General subjects Vol. 1863; no. 6; pp. 1158 - 1166
• Main Authors: Fan, Xiaobo, Xu, Wei, Han, Jinsong, Jiang, Xinglu, Wink, Michael, Wu, Guoqiu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2019
• Subjects: Anti-Bacterial Agents - chemistry; Antimicrobial Cationic Peptides - chemistry; Antimicrobial Cationic Peptides - genetics; antimicrobial peptides; antimicrobial properties; antimicrobial proteins; bacteria; Bacteria - growth & development; Bacteria - isolation & purification; Bacterial discrimination; bacterial growth; buffers; calcium; cell membranes; Clinic isolate; Colorimetry; culture media; discriminant analysis; Escherichia coli; Green fluorescent protein; Green Fluorescent Proteins - chemistry; Green Fluorescent Proteins - genetics; Humans; ions; lipopolysaccharides; Membrane-penetrating protein; minimum inhibitory concentration; quartz crystal microbalance; Quartz Crystal Microbalance Techniques; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; MIC; QCM-D; Bacterial discrimination; LDA; Colorimetry; LPS; GFP; AMPs; IGP; Green fluorescent protein; Clinic isolate; Membrane-penetrating protein; CLSM; TEM; PCR

Antimicrobial peptides killed bacteria through intercalating into the bacterial membrane. Their antimicrobial efficiencies varied in bacterial species and were affected by ion strength in the culture medium. A recombinant IGP protein consisted of an antimicrobial peptide, Ib-AMP4 fused with the Gree...