Covalently bound DNA on naked iron oxide nanoparticles: Intelligent colloidal nano-vector for cell transfection

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 11; pp. 2802 - 2810
• Main Authors: Magro, Massimiliano, Martinello, Tiziana, Bonaiuto, Emanuela, Gomiero, Chiara, Baratella, Davide, Zoppellaro, Giorgio, Cozza, Giorgio, Patruno, Marco, Zboril, Radek, Vianello, Fabio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017
• Subjects: Biophysical Phenomena; chemical bonding; colloids; Colloids - chemistry; DNA - chemistry; DNA - genetics; DNA chemisorption; Ferric Compounds - chemistry; fluorescence; Fluorescent nanoparticles; Gene Transfer Techniques; genes; Genetic Vectors; Green fluorescent protein (GFP); horses; Humans; hydrodynamics; light scattering; maghemite; magnetic fields; Magnetic nanoparticles; Mesenchymal stem cell; microscopy; Microscopy, Electron, Transmission; nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Particle Size; plasmids; Plasmids - chemistry; Plasmids - genetics; spectroscopy; stem cells; toxicity; Transfection; Transfection - methods; zeta potential; Mesenchymal stem cell; Fluorescent nanoparticles; DNA chemisorption; Transfection; Magnetic nanoparticles; Green fluorescent protein (GFP)
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2017.07.025

Conversely to common coated iron oxide nanoparticles, novel naked surface active maghemite nanoparticles (SAMNs) can covalently bind DNA. Plasmid (pDNA) harboring the coding gene for GFP was directly chemisorbed onto SAMNs, leading to a novel DNA nanovector (SAMN@pDNA). The spontaneous internalizati...