Deregulation of Bmi-1 is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma

• Published in: Biochimica et biophysica acta Vol. 1840; no. 12; pp. 3285 - 3291
• Main Authors: Chang, Boyang, Li, Su, He, Qianting, Liu, Zhonghua, Zhao, Luodan, Zhao, Tingting, Wang, Anxun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2014
• Subjects: Bmi-1; carcinogenesis; carcinoma; Metastasis; prognosis; Salivary adenoid cystic carcinoma; stem cells; vimentin; Salivary adenoid cystic carcinoma; Bmi-1; Metastasis
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2014.08.005

Bmi-1 had been found to involve in self renewal of stem cells and tumorigenesis in various malignancies. In this study, we investigated the role of Bmi-1 in the development of salivary adenoid cystic carcinoma (SACC). At first, we confirmed that the deregulation of Bmi-1 was a frequent event in SACC; up-regulation of Bmi-1 was correlated with clinical stages, vital status and distant metastasis and associated with reduced overall survival and disease free survival. SACC-LM cells, higher migration and invasion abilities, elevated the expression of Bmi-1 protein, epithelial-mesenchymal transition (EMT) related proteins (Snail, Slug and Vimentin) and cancer stem cells (CSCs) related proteins (ABCG2, Notch, ALDH-1, Oct-4, Nanog and Epcam) compared to the SACC-83 cells (lower migration and invasion abilities). The migration and invasion abilities were inhibited in SACC-LM cells upon Bmi-1 knockdown. Meanwhile, Bmi-1 knockdown resulted in simultaneous loss of stem cell markers and EMT markers in SACC-LM cells. Our studies confirm that Bmi-1 deregulation plays an important role in the development of SACC and contributes to the migration and the invasion abilities of SACC, which is involved in EMT and CSCs. To our knowledge, this is the first study revealing that Bmi-1 deregulation is associated with enhanced migration, invasion and poor prognosis in salivary adenoid cystic carcinoma. •Bmi-1 deregulation plays an important role in the development of SACC.•Bmi-1 overexpression is related to the migration and invasion of SACC.•Bmi-1 knockdown inhibits the migration and invasion of SACC.•Bmi-1 deregulation was involved in EMT and CSCs in the metastasis of SACC.