The use of calorimetry in the biophysical characterization of small molecule alkaloids binding to RNA structures

• Published in: Biochimica et biophysica acta Vol. 1860; no. 5; pp. 930 - 944
• Main Authors: Kumar, Gopinatha Suresh, Basu, Anirban
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2016
• Subjects: alkaloids; Alkaloids - chemistry; Binding Sites; biocalorimetry; Calorimetry; Calorimetry - instrumentation; Calorimetry - methods; differential scanning calorimetry; drugs; instrumentation; Kinetics; Natural alkaloids; Poly A - chemistry; RNA targeted drug design; RNA, Double-Stranded - chemistry; RNA, Transfer - chemistry; Thermodynamics; titration; transfer RNA; Natural alkaloids; Calorimetry; RNA targeted drug design

RNA has now emerged as a potential target for therapeutic intervention. RNA targeted drug design requires detailed thermodynamic characterization that provides new insights into the interactions and this together with structural data, may be used in rational drug design. The use of calorimetry to ch...