Prolyl hydroxylation in elastin is not random

• Published in: Biochimica et biophysica acta Vol. 1860; no. 10; pp. 2169 - 2177
• Main Authors: Schmelzer, Christian E.H., Nagel, Marcus B.M., Dziomba, Szymon, Merkher, Yulia, Sivan, Sarit S., Heinz, Andrea
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2016
• Subjects: Animals; bioinformatics; biomechanics; Cattle; Chickens; Collagen - genetics; Collagen - metabolism; Elastic fiber; elastin; Elastin - genetics; Elastin - metabolism; Extracellular matrix; Humans; hydroxylation; Hydroxylation - genetics; Hydroxylysine; Hydroxyproline; intervertebral disks; lysine; Lysine - chemistry; Lysine - metabolism; Mass spectrometry; Organ Specificity; procollagen-proline dioxygenase; proline; Proline - metabolism; Prolyl 4-hydroxylase; Prolyl Hydroxylases - chemistry; Prolyl Hydroxylases - genetics; Protein Processing, Post-Translational - genetics; Swine; PP-II; P4H; MS; Hydroxyproline; PTM; mRNA; ACN; ECM; TFA; Tris; SD; TE; Prolyl 4-hydroxylase; PE; IVD; Extracellular matrix; Elastic fiber; HyK; Hydroxylysine; HD; LH; Mass spectrometry; HPLC; HyP

This study aimed to investigate the prolyl and lysine hydroxylation in elastin from different species and tissues. Enzymatic digests of elastin samples from human, cattle, pig and chicken were analyzed using mass spectrometry and bioinformatics tools. It was confirmed at the protein level that elast...