Biophysical characterization of E. coli TolC interaction with the known blocker hexaamminecobalt

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 11; pp. 2702 - 2709
• Main Authors: Gilardi, A., Bhamidimarri, S.P., Brönstrup, M., Bilitewski, U., Marreddy, R.K.R., Pos, K.M., Benier, L., Gribbon, P., Winterhalter, M., Windshügel, B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017
• Subjects: antibacterial properties; antibiotic resistance; Bacterial Outer Membrane Proteins - antagonists & inhibitors; Bacterial Outer Membrane Proteins - chemistry; Bacterial Outer Membrane Proteins - drug effects; Bacterial Outer Membrane Proteins - genetics; Biophysical Phenomena; cations; Cobalt - pharmacology; dissociation; Drug Resistance, Multiple, Bacterial - drug effects; Drug Resistance, Multiple, Bacterial - genetics; drugs; electric power; Electrophysiology; erythromycin; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli Proteins - antagonists & inhibitors; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Gene Expression Regulation, Bacterial - drug effects; Hexaamminecobalt; ligands; Membrane Transport Proteins - chemistry; Membrane Transport Proteins - genetics; minimum inhibitory concentration; outer membrane proteins; Surface Plasmon Resonance; TolC; transporters; CCCP; Hexaamminecobalt; SPR; DMSO; TolC; AMR; MIC; Electrophysiology; RND; OMF; Surface plasmon resonance; HC
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2017.07.014

The tripartite efflux pump AcrAB-TolC in E. coli is involved in drug resistance by transporting antibiotics out of the cell. The outer membrane protein TolC can be blocked by various cations, including hexaamminecobalt, thereby TolC represents a potential target for reducing antimicrobial resistance...