Structure-based design and application of an engineered glutathione transferase for the development of an optical biosensor for pesticides determination - Chronopoulou, Evangelia G.; Vlachakis, Dimitrios; Papageorgiou, Anastassios C.; Ataya, Farid S.; Labrou, Nikolaos E. (Biochimica et biophysica acta. General subjects, 2019)
Keywords: binding properties; binding sites; Binding Sites - genetics; Biosensing Techniques - methods; Biosensor; biosensors; Catalysis; catalytic activity; Catalytic Domain - genetics; Directed Molecular Evolution - methods; Endosulfan - analysis; Endosulfan - isolation & purification; Environmental Monitoring - methods; Environmental Pollutants - analysis; Environmental Pollutants - isolation & purification; Enzymes, Immobilized - genetics; Enzymes, Immobilized - metabolism; Glutathione - metabolism; Glutathione transferase; Glutathione Transferase - chemistry; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Glycine max - enzymology; Glycine max - genetics; hydrophobicity; mutagenesis; Mutagenesis, Site-Directed; Mutant Proteins - metabolism; mutants; pesticides; Pesticides - analysis; Pesticides - isolation & purification; Pesticides determination; Phaseolus - enzymology; Phaseolus - genetics; phenolsulfonphthalein; Protein engineering; Protein Engineering - methods; protons; Reproducibility of Results; screening; standard deviation; Structure-Activity Relationship; xenobiotics; α-Endosulfan; H-site; RDS; Protein engineering; PTMOS; GST; α-Endosulfan; ANNs; TEOS; PEG; Glutathione transferase; G-site; Biosensor; MD; Pesticides determination; PvGmGSTUG; GSH