Pneumolysin-induced autophagy contributes to inhibition of osteoblast differentiation through downregulation of Sp1 in human osteosarcoma cells

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 11; pp. 2663 - 2673
• Main Authors: Kim, Jinwook, Lee, Hee-Weon, Rhee, Dong Kwon, Paton, James C., Pyo, Suhkneung
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017
• Subjects: AMPK; arthritis; Autophagy; Autophagy - drug effects; Autophagy - genetics; Bacterial Proteins - administration & dosage; Bacterial Proteins - metabolism; Cell Differentiation - drug effects; flow cytometry; Gene Expression Regulation, Neoplastic - drug effects; genetic markers; Humans; mechanism of action; mTOR; Osteoblast; osteoblasts; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteoblasts - pathology; Osteogenesis - drug effects; osteomyelitis; osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - pathology; pathogenesis; Pneumolysin; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; RNA interference; ROS; small interfering RNA; Sp1; Sp1 Transcription Factor - genetics; Streptococcus pneumoniae; Streptococcus pneumoniae - chemistry; Streptococcus pneumoniae - pathogenicity; Streptolysins - administration & dosage; Streptolysins - metabolism; TOR Serine-Threonine Kinases - metabolism; virulence; Western blotting; Pneumolysin; Sp1; ROS; Osteoblast; mTOR; AMPK; Autophagy

The 53kDa protein pneumolysin (PLY) is the main virulence factor of Streptococcus pneumoniae, a leading cause of invasive pneumococcal diseases. PLY forms pores in cholesterol-containing membranes, thereby interfering with the function of cells. Bone destruction is a serious matter in chronic inflam...