An Integrative Approach to Determine 3D Protein Structures Using Sparse Paramagnetic NMR Data and Physical Modeling

• Published in: Frontiers in molecular biosciences Vol. 8; p. 676268
• Main Authors: Gaalswyk, Kari, Liu, Zhihong, Vogel, Hans J, MacCallum, Justin L
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 12.08.2021
• Subjects: calmodulin; integrative structural biology; modeling; Molecular Biosciences; NMR; paramagnetic relaxation enhancement; protein structure; protein structure; NMR; paramagnetic relaxation enhancement; modeling; integrative structural biology; calmodulin

Paramagnetic nuclear magnetic resonance (NMR) methods have emerged as powerful tools for structure determination of large, sparsely protonated proteins. However traditional applications face several challenges, including a need for large datasets to offset the sparsity of restraints, the difficulty...