Engineering bacterial translation initiation — Do we have all the tools we need?

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 11; pp. 3060 - 3069
• Main Authors: Vigar, Justin R.J., Wieden, Hans-Joachim
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017
• Subjects: bacteria; Bacteria - genetics; Bacteria - metabolism; Codon, Initiator - genetics; Codon, Initiator - metabolism; Escherichia coli; gene expression; Gene Expression Regulation, Bacterial; ligands; messenger RNA; Peptide Chain Initiation, Translational - genetics; Protein Biosynthesis - genetics; Protein Engineering - methods; protein synthesis; Rational design; regulatory sequences; Riboregulation; Ribosome engineering; ribosomes; Ribosomes - metabolism; RNA engineering; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-binding proteins; Synthetic biology; synthetic genes; translation (genetics); Translation initiation; Synthetic biology; Rational design; Ribosome engineering; RNA engineering; Translation initiation; Riboregulation

Reliable tools that allow precise and predictable control over gene expression are critical for the success of nearly all bioengineering applications. Translation initiation is the most regulated phase during protein biosynthesis, and is therefore a promising target for exerting control over gene ex...