A hydrogen peroxide safety valve: The reversible phosphorylation of catalase from the freeze-tolerant North American wood frog, Rana sylvatica

• Published in: Biochimica et biophysica acta Vol. 1860; no. 3; pp. 476 - 485
• Main Authors: Dawson, Neal J., Storey, Kenneth B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2016
• Subjects: AMP-activated protein kinase; Animals; Antioxidant; blood flow; cAMP-dependent protein kinase; catalase; Catalase - metabolism; cold tolerance; Enzyme Stability; Freeze tolerance; Freezing; frogs; hydrogen peroxide; Hydrogen Peroxide - metabolism; Ischemia; Kinetics; Lithobates sylvaticus; Male; Oxidative stress; oxygen; phosphoprotein phosphatase; Phosphorylation; Rana sylvatica; Ranidae - metabolism; Reversible protein phosphorylation; skeletal muscle; superoxide dismutase; Oxidative stress; Km; DTT; cGMP; Freeze tolerance; cAMP; H2O2; EGFR; Erk1; Ischemia; GSK3; PDGFR; Tm; NBT; CAMK; KPi; Vmax; PKA; TBST; PKC; PP2A; Akt; DEPC; EDTA; MAPK; Antioxidant; PKG; PVDF; Cdc2; EGTA; PP1; SDS-PAGE; Cdk5; PP2C; Lck; PMSF; PP2B; ROS; Rana sylvatica; AMPK; DEAE; Reversible protein phosphorylation

The North American wood frog, Rana sylvatica, endures whole body freezing while wintering on land and has developed multiple biochemical adaptations to elude cell/tissue damage and optimize its freeze tolerance. Blood flow is halted in the frozen state, imparting both ischemic and oxidative stress o...