Characterization of β-lactamase activity using isothermal titration calorimetry

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 8; pp. 2031 - 2038
• Main Authors: Wang, Wen-Jing, Wang, Qian, Zhang, Ye, Lu, Rui, Zhang, Yi-Lin, Yang, Ke-Wu, Lei, Jin-E, He, Yuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2017
• Subjects: Anti-Bacterial Agents - pharmacology; antibiotic resistance; Antimicrobial resistance; bacteria; benzylpenicillin; beta-lactamase; beta-Lactamase Inhibitors - pharmacology; beta-Lactamases - metabolism; calorimetry; Calorimetry - methods; cefazolin; enzymatic hydrolysis; enzyme kinetics; Escherichia coli; heat; hydrolysis; imipenem; Inhibitor; Isothermal titration calorimetry; Microbial Sensitivity Tests; screening; spectrophotometers; Spectrophotometry; Steady-state kinetics; Thermodynamics; titration; β-lactamase; Thermodynamics; Inhibitor; Isothermal titration calorimetry; Antimicrobial resistance; β-lactamase; Steady-state kinetics
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2017.04.011

Hydrolysis of β-lactam antibiotic by β-lactamase is the most common mechanism of β-lactam resistance in clinical isolates. Timely detection and characterization of β-lactamases are therefore of utmost biomedical importance. Conventional spectrophotometric method is time-consuming and cannot provide...