High yield expression and purification of equilibrative nucleoside transporter 7 (ENT7) from Arabidopsis thaliana - Girke, Christopher; Arutyunova, Elena; Syed, Maria; Traub, Michaela; Möhlmann, Torsten; Lemieux, M. Joanne (Biochimica et biophysica acta, 2015)
Keywords: Animals; Arabidopsis - metabolism; Arabidopsis thaliana; ENT7; Equilibrative Nucleoside Transport Proteins - genetics; Equilibrative Nucleoside Transport Proteins - isolation & purification; Equilibrative Nucleoside Transport Proteins - metabolism; Equilibrative nucleoside transporter; Fluorescence size exclusion chromatography; FSEC; gel chromatography; green fluorescent protein; Komagataella pastoris; mammals; Membrane transport; Microscale thermophoresis; MST; Nucleobase; Nucleoside; Oocytes; pyrimidine nucleosides; Recombinant expression; recombinant proteins; Recombinant Proteins - biosynthesis; Reconstitution; transporters; Xenopus laevis; Xenopus laevis - genetics; Arabidopsis thaliana; Recombinant expression; Fluorescence size exclusion chromatography; Equilibrative nucleoside transporter; Membrane transport; Nucleoside; Reconstitution; FSEC; Nucleobase; ENT7; Microscale thermophoresis; MST