Targeted Ablation of the Murine alpha-Tropomyosin Gene

• Published in: Circulation research Vol. 81; no. 6; pp. 1005 - 1010
• Main Authors: Blanchard, Edward M, Iizuka, Kenji, Christe, Michael, Conner, David A, Geisterfer-Lowrance, Anja, Schoen, Frederick J, Maughan, David W, Seidman, Christine E, Seidman, J.G
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD American Heart Association, Inc 01.12.1997; Lippincott; Lippincott Williams & Wilkins Ovid Technologies
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Contractile proteins; Fundamental and applied biological sciences. Psychology; Gene Targeting; Holoproteins; Male; Mice; Mice, Knockout; Myocardium - pathology; Proteins; Tropomyosin - genetics; Tropomyosin - physiology; Embryo; Molecular form; Myofibril; Tropomyosin; Papillary muscle; Rodentia; Site directed mutagenesis; Contractile protein; Strain specificity; Striated muscle; Heterozygosity; Vertebrata; Mammalia; Mouse; Mutation; Performance

We created a mouse that lacks a functional alpha-tropomyosin gene using gene targeting in embryonic stem cells and blastocyst-mediated transgenesis. Homozygous alpha-tropomyosin "knockout" mice die between embryonic day 9.5 and 13.5 and lack alpha-tropomyosin mRNA. Heterozygous alpha-tropo...