Characterization of NTera2/D1 cells as a model system for the investigation of cannabinoid function in human neurons and astrocytes

• Published in: Journal of neuroscience research Vol. 89; no. 10; pp. 1685 - 1697
• Main Authors: Goodfellow, Catherine E., Graham, Scott E., Dragunow, Mike, Glass, Michelle
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2011
• Subjects: Astrocytes; Astrocytes - cytology; Astrocytes - drug effects; Astrocytes - pathology; Brain; Brain Chemistry - drug effects; Brain Chemistry - genetics; Brain Chemistry - physiology; cannabinoid receptor CB1; cannabinoid receptor CB2; Cannabinoid Receptor Modulators - genetics; Cannabinoid Receptor Modulators - metabolism; Cannabinoid receptors; Cell culture; Cell Culture Techniques - methods; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Line, Tumor; Central nervous system; Cyclic AMP; endocannabinoids; Enzymes; Gene expression; Glial cells; Humans; Immunocytochemistry; Metabolism; Nervous system; Neural stem cells; Neuronal-glial interactions; Neurons; Neurons - cytology; Neurons - drug effects; Neurons - pathology; Neurotrophin 2; Phenotype; Polymerase chain reaction; Teratocarcinoma - chemistry; Teratocarcinoma - genetics; Teratocarcinoma - pathology

The limited availability and potential to culture primary human brain cells means that there is still a need for cell lines that reliably model human neurons and glial cells. The human‐derived NTera2/D1 (NT2) cell line is a promising tool from which both neuronal (NT2N) and astrocytic (NT2A) cells c...