Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast, Aureobasidium pullulans

• Published in: Molecular biology of the cell Vol. 35; no. 4; p. br10
• Main Authors: Petrucco, Claudia A, Crocker, Alex W, D'Alessandro, Alec, Medina, Edgar M, Gorman, Olivia, McNeill, Jessica, Gladfelter, Amy S, Lew, Daniel J
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.04.2024
• Series: A Highlights from
• Subjects: Ascomycota - metabolism; Aureobasidium; Cytoskeleton; Saccharomyces cerevisiae; Special Issue on Evolutionary Cell Biology
• ISSN: 1059-1524; 1939-4586
• DOI: 10.1091/mbc.E23-10-0388

is a ubiquitous fungus with a wide variety of morphologies and growth modes including "typical" single-budding yeast, and interestingly, larger multinucleate yeast than can make multiple buds in a single cell cycle. The study of promises to uncover novel cell biology, but currently tools are lacking to achieve this goal. Here, we describe initial components of a cell biology toolkit for , which is used to express and image fluorescent probes for nuclei as well as components of the cytoskeleton. These tools allowed live-cell imaging of the multinucleate and multibudding cycles, revealing highly synchronous mitoses in multinucleate yeast that occur in a semiopen manner with an intact but permeable nuclear envelope. These findings open the door to using this ubiquitous polyextremotolerant fungus as a model for evolutionary cell biology.