Natural Cytotoxic Reactivity of Human Lymphocytes Against a Myeloid Cell Line: Characterization of Effector Cells

Using a series of techniques to identify and deplete various peripheral blood lymphocyte subpopulations, we studied the cytotoxic reactivity of normal individuals against the myeloid cell line K-562 in a 4-hr 51chromium-release assay. Depletion of lymphocytes bearing complement receptors had a varia...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 118; no. 1; pp. 355 - 361
Main Authors West, William H, Cannon, Grace B, Kay, H. David, Bonnard, Guy D, Herberman, Ronald B
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.01.1977
Subjects
Antigen-Antibody Complex
Binding Sites
Bone Marrow - immunology
Bone Marrow Cells
Cell Line
Complement System Proteins
Cytotoxicity Tests, Immunologic
Humans
Immune Adherence Reaction
Immunity, Cellular
Immunoglobulin Fc Fragments
Immunoglobulin G
Leukemia, Myeloid - immunology
Lymphocytes - immunology
Macrophages - immunology
T-Lymphocytes - immunology
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Summary:Using a series of techniques to identify and deplete various peripheral blood lymphocyte subpopulations, we studied the cytotoxic reactivity of normal individuals against the myeloid cell line K-562 in a 4-hr 51chromium-release assay. Depletion of lymphocytes bearing complement receptors had a variable, usually negligible effect on cytotoxicity. In contrast, depletion of lymphocytes bearing Fc receptors abrogated target cell lysis. Separation of lymphocytes with high-affinity binding of sheep red blood cells (SRBC) evidenced by rosette formation at 29 degrees C yielded a population of rosette-forming cells containing few cytotoxic cells, whereas separation of total E-RFC under optimal rosetting conditions produced a rosette fraction containing a major proportion of the effector cells. These data indicate that the cytotoxic lymphocyte in this system is Fc receptor positive, largely complement receptor negative, and may possess low density or low affinity receptors for SRBC.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.118.1.355