COMPARISON OF PRIMARY HUMAN HEPATOCYTES AND HEPATOMA CELL LINE HEPG2 WITH REGARD TO THEIR BIOTRANSFORMATION PROPERTIES

• Published in: Drug metabolism and disposition Vol. 31; no. 8; pp. 1035 - 1042
• Main Authors: WILKENING, Stefan, STAHL, Frank, BADER, Augustinus
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Pharmacology and Experimental Therapeutics 01.08.2003
• Subjects: 7-Alkoxycoumarin O-Dealkylase - biosynthesis; Benzo(a)pyrene - adverse effects; Benzo(a)pyrene - metabolism; Benzo(a)pyrene - pharmacokinetics; Biological and medical sciences; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Comet Assay; Cytochrome P-450 CYP1A1 - biosynthesis; Cytoskeleton - ultrastructure; Dimethylnitrosamine - adverse effects; Dimethylnitrosamine - metabolism; Dimethylnitrosamine - pharmacokinetics; Dose-Response Relationship, Drug; Enzyme Induction - drug effects; Enzyme Induction - genetics; Female; Gene Expression - drug effects; Gene Expression - genetics; General aspects (metabolism, cell proliferation, established cell line...); Hepatocytes - drug effects; Hepatocytes - metabolism; Humans; Imidazoles - pharmacokinetics; Inactivation, Metabolic - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Male; Medical sciences; Middle Aged; Mutagens - adverse effects; Mutagens - metabolism; Mutagens - pharmacokinetics; Reverse Transcriptase Polymerase Chain Reaction; Tumor cell; Tumors; Human; Enzyme; Liver; Hepatic disease; Hepatocellular carcinoma; Malignant tumor; Metabolism; In vitro; Hepatocyte; Biotransformation; Established cell line; Digestive diseases; Drug-metabolizing enzyme; Tumor cell; Comparative study

Cultures of primary hepatocytes and hepatoma cell line HepG2 are frequently used in in vitro models for human biotransformation studies. In this study, we characterized and compared the capacity of these model systems to indicate the presence of different classes of promutagens. Genotoxic sensitivit...