Optimization of technical conditions for the transformation of Pediococcus acidilactici P60 by electroporation

• Published in: Plasmid Vol. 58; no. 1; pp. 44 - 50
• Main Authors: Rodríguez, M. Carmen, Alegre, M. Teresa, Mesas, Juan M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.07.2007
• Subjects: DNA, Bacterial - genetics; Electroporation; Pediococci; Pediococcus - genetics; Pediococcus acidilactici; Plasmids; Transformation, Bacterial; Plasmids; Electroporation; Pediococci

Previously reported techniques for the electrotransfer of foreign DNA into pediococci yield only a small number of transformants/μg DNA, especially when using undomesticated strains. This study reports an improved protocol for the electrotransformation of pediococci, based on trials using Pediococcu...