Effect of intramuscular injection of hepatocyte growth factor plasmid DNA with electroporation on bleomycin-induced lung fibrosis in rats

• Published in: Chinese medical journal Vol. 120; no. 16; pp. 1432 - 1437
• Main Authors: Long, Xiang, Xiong, Sheng-dao, Xiong, Wei-ning, Xu, Yong-jian
• Format: Journal Article
• Language: English
• Published: China Institute of Pulmonary Disease, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China 20.08.2007
• Subjects: Animals; Bleomycin - toxicity; Cyclooxygenase 2 - genetics; Electroporation; Genetic Therapy; Hepatocyte Growth Factor - genetics; Hydroxyproline - analysis; Injections, Intramuscular; Lung - chemistry; Male; Muscle, Skeletal - metabolism; Plasmids; Pulmonary Fibrosis - chemically induced; Pulmonary Fibrosis - therapy; Rats; Rats, Wistar; RNA, Messenger - analysis; Transforming Growth Factor beta1 - analysis; Transforming Growth Factor beta1 - genetics; gene therapy; electroporation; hepatocyte growth factor; pulmonary fibrosis

So far, there is no efficient treatment for pulmonary fibrosis. The objective of this study was to determine whether intramuscular injection of the hepatocyte growth factor (HGF) plasmid DNA by in vivo electroporation could prevent bleomycin-induced pulmonary fibrosis in rats, and to investigate the...