Hydrosilated silica-based columns: The effects of mobile phase and temperature on dual hydrophilic-reversed-phase separation mechanism of phenolic acids

• Published in: Journal of Chromatography A Vol. 1228; pp. 125 - 134
• Main Authors: Soukup, Jan, Jandera, Pavel
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 09.03.2012
• Subjects: Acetonitriles - chemistry; Cholesterol - chemistry; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Chromatography, Reverse-Phase; HILIC; Hydrophobic and Hydrophilic Interactions; Hydrosilated silica; Hydroxybenzoates - isolation & purification; Mobile phase; Phenolic acids; Silicon Dioxide - chemistry; Temperature; Temperature effects; Mobile phase; Temperature effects; HILIC; Phenolic acids; Hydrosilated silica

The effects of mobile phase composition and of temperature on the retention behavior of phenolic acids were studied on 4 hydrosilated (type C silica) based columns in buffered aqueous acetonitrile, both in the aqueous normal phase (HILIC) and in the reversed-phase mobile phase range. The UDC cholest...