Melanin Biosynthesis Inhibition Effects of Ginsenoside Rb2 Isolated from Panax ginseng Berry

• Published in: Journal of microbiology and biotechnology Vol. 25; no. 12; pp. 2011 - 2015
• Main Authors: Lee, Dae Young, Jeong, Yong Tae, Jeong, Sang Chul, Lee, Mi Kyoung, Min, Jin Woo, Lee, Jae Won, Kim, Geum Soog, Lee, Seung Eun, Ahn, Young Sup, Kang, Hee Cheol, Kim, Jin Hee
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.12.2015
• Subjects: Animals; Cell Line; Ginsenosides - chemistry; Ginsenosides - isolation & purification; Ginsenosides - metabolism; Melanins - analysis; Melanins - antagonists & inhibitors; Melanocytes - drug effects; Monophenol Monooxygenase - analysis; Monophenol Monooxygenase - antagonists & inhibitors; Panax - chemistry; Spectrum Analysis; Zebrafish; 생물학; melanin biosysnthesis; zebrafish; melan-a cell; Panax ginseng; ginsenoside Rb2
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1505.05069

Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, (1)H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.