Biosynthesized gold and silver nanoparticles by aqueous fruit extract of Chaenomeles sinensis and screening of their biomedical activities

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 46; no. 3; pp. 599 - 606
• Main Authors: Oh, Keun Hyun, Soshnikova, Veronika, Markus, Josua, Kim, Yeon Ju, Lee, Sang Chul, Singh, Priyanka, Castro-Aceituno, Verónica, Ahn, Sungeun, Kim, Dong Hyun, Shim, Yeon Jae, Kim, Yu Jin, Yang, Deok Chun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.05.2018; Taylor & Francis Ltd
• Subjects: Antimicrobial activity; antioxidant activity; Biocompatibility; Biological activity; Biomedical materials; Breast cancer; Cancer; Chaenomeles sinensis; Cytotoxicity; E coli; Electron microscopy; Emission analysis; Gold; gold nanoparticles; Macrophages; Nanoparticles; Nanotechnology; Silver; silver nanoparticles; Spectroscopy; Toxicity; Transmission electron microscopy; Tumor cell lines; X-ray diffraction; Chaenomeles sinensis; antimicrobial activity; silver nanoparticles; gold nanoparticles; antioxidant activity
• ISSN: 2169-1401; 2169-141X; 2169-141X
• DOI: 10.1080/21691401.2017.1332636

The design of mild and non-toxic synthesis of metallic nanoparticles is a topical subject in the nanotechnology field. The objective of this present study is to screen the bioactivity of biosynthesized nanoparticles by aqueous fruit extract of Chaenomeles sinensis. The reducing and stabilizing ability of C. sinensis to fabricate gold (Cs-AuNps) and silver (Cs-AgNps) nanoparticles was confirmed by UV-visible (UV-Vis) spectroscopy at 562 nm and 477 nm, respectively. The field-emission transmission electron microscopy (FE-TEM) and X-ray diffraction analysis (XRD) verify the nano-scale morphology and crystallinity of Cs-AuNps (20-40 nm) and Cs-AgNps (5-20 nm). Furthermore, we evaluated the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity, antimicrobial activity against Staphylococcus aureus and Escherichia coli and cytotoxicity against breast cancer cells. The results showed that Cs-AuNps (IC 50 : 725.93 μg/mL) displayed superior inhibitory activities on DPPH than Cs-AuNps. The biosynthesized Cs-AuNps successfully inhibited the growth of pathogenic bacteria S. aureus (ATCC 6538) and E. coli (BL21). The cytotoxic effect of Cs-AuNps and Cs-AgNps was evaluated in murine macrophage (RAW264.7) and human breast cancer cell lines (MCF7) by MTT assay. Thus, the present study explores the biomedical applications of gold and silver nanoparticles synthesized by C. sinensis.