A male germ cell assay and supporting somatic cells: its application for the detection of phase specificity of genotoxins in vitro

• Published in: Journal of toxicology and environmental health. Part B, Critical reviews Vol. 23; no. 3; pp. 91 - 106
• Main Authors: Habas, Khaled, Brinkworth, Martin H., Anderson, Diana
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.04.2020; Taylor & Francis Ltd
• Subjects: Animal research; Anticancer properties; Antitumor agents; Apoptosis; Deoxyribonucleic acid; DNA; DNA damage; Fertility; Genotoxicity; Germ cells; In vivo methods and tests; Male germ cell; Mammals; Mutagenicity; Mutation; Offspring; phase specificity; Purification; Regulatory agencies; Sedimentation; Somatic cells; Spermatogenesis; STAPUT gravity sedimentation; Stem cell transplantation; Stem cells; supporting somatic cells; Male germ cell; phase specificity; supporting somatic cells; STAPUT gravity sedimentation; DNA damage; Apoptosis

Male germ stem cells are responsible for transmission of genetic information to the next generation. Some chemicals exert a negative impact on male germ cells, either directly, or indirectly affecting them through their action on somatic cells. Ultimately, these effects might inhibit fertility, and...