Discovery, gene modification, and optimization of fermentation of an enduracidin-producing strain

• Published in: Journal of asian natural products research Vol. 20; no. 7; pp. 633 - 648
• Main Authors: Zhong, Tian-Hua, Zeng, Xian-Ming, Zhang, Yong-Hong, Chan, Zhu-Hua, Luo, Zhu-Hua, Yang, Xian-Wen, Xu, Wei
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.07.2018; Taylor & Francis Ltd
• Subjects: Amino Acids - pharmacology; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Durability; Enduracidin; Fermentation; fermentation optimization; gene mutation; Genetic modification; Gram-positive bacteria; Industrial production; marine microorganisms; Molecular Structure; Mutation; Nitrogen - metabolism; Optimization; Peptides, Cyclic - biosynthesis; Peptides, Cyclic - chemistry; Peptides, Cyclic - pharmacology; Streptomyces - drug effects; Streptomyces - genetics; Streptomyces - metabolism; Temperature; Yield; Enduracidin; marine microorganisms; gene mutation; fermentation optimization
• ISSN: 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2018.1451517

Enduracidin significantly inhibits Gram-positive bacteria and had been widely used in many fields. However, as the poor technology for production of enduracidin and its scarcity, identification of novel strategies for production of enduracidin is important. Our group developed two methods to improve the yield of the production of enduracidin. The yield of enduracidin was increased by three- to fivefold. The highest yields of enduracidin A and enduracidin B achieved were 63.7 and 82.13 mg/ml. Thus, our results might provide a new reference method for the industrial production of enduracidin.