Detection of biomarkers using a novel proximity extension assay in patients with ST-elevation myocardial infarction

• Published in: Thrombosis research Vol. 172; pp. 21 - 28
• Main Authors: Kulasingam, Archana, Hvas, Anne-Mette, Grove, Erik Lerkevang, Funck, Kristian Løkke, Kristensen, Steen Dalby
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.12.2018
• Subjects: Acute myocardial infarction; Aged; Biomarkers; Biomarkers - blood; Cell Adhesion; Coronary artery disease; Female; Fibrinolysis; Hemostasis; Humans; Inflammation; Inflammation - blood; Male; Middle Aged; Percutaneous Coronary Intervention; Prospective Studies; Proteomics; ST Elevation Myocardial Infarction - blood; ST Elevation Myocardial Infarction - surgery; CPA1; MCP-1; CPB1; tPA; RARRES2; MEPE; Inflammation; Coronary artery disease; CHIT1; PDGF subunit A; IGFBP-1; IGFBP-2; MMP-2; MMP-3; Proteomics; STEMI; Biomarkers; PAI; PI3; Acute myocardial infarction; TIMP4
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/j.thromres.2018.10.011

ST-elevation myocardial infarction (STEMI) involves inflammation, activation of platelets, coagulation and changes in fibrinolysis as well as remodelling of myocardial tissue after STEMI. Recently, new proximity extension assays including panels of biomarkers for cardiovascular disease have been developed. This study investigates a wide range of cardiovascular protein biomarkers in the acute phase of STEMI compared with the stable phase three months after STEMI. We hypothesized that major changes in inflammation, haemostasis, tissue remodelling, and proteolysis are prevalent. This was a prospective, observational study including 48 STEMI patients (mean age 60 ± 12 years, 79% men) treated with primary percutaneous coronary intervention (PPCI). Blood samples were obtained immediately prior to PPCI and again three months later. Levels of 92 biomarkers reflecting inflammation, immune response, cell adhesion, haemostasis, fibrinolysis, tissue remodelling, and proteolysis were assessed using a proximity extension assay (Olink® CARDIOVASCULAR III). When comparing the acute phase of STEMI with the stable phase three months later, a total of 29 biomarkers differed significantly after Bonferroni correction (p < 0.0005). In the acute phase of STEMI, we found an overall increase of biomarkers reflecting immune and inflammatory response, cell adhesion, and haemostasis. Biomarkers reflecting tissue remodelling and proteolysis were increased at three months follow-up compared with the acute phase. Out of the 29 biomarkers, six biomarkers did not confirm our predefined hypotheses. Using a novel proximity extension assay technique, we detected changes in several biomarkers when comparing the acute phase with three months follow-up in patients with STEMI. These biomarkers may play important roles in the pathogenesis of STEMI. •The biomarker panel reflects inflammation, haemostasis, remodelling, and proteolysis.•Significant changes in 29 biomarkers when comparing acute STEMI with follow-up phase•Novel findings of changes in some important biomarkers•Our hypothesis-generating findings should be validated in future studies.