The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell

• Published in: Nutrition and cancer Vol. 72; no. 7; pp. 1231 - 1242
• Main Authors: Hoca, Mustafa, Becer, Eda, Kabadayı, Hilal, Yücecan, Sevinç, Vatansever, Hafize Seda
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 02.10.2020; Taylor & Francis Ltd
• Subjects: Antibodies; Anticancer properties; Antigens, CD - metabolism; Antineoplastic Agents - pharmacology; Cadherins; Cadherins - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Cytotoxicity; Epithelial-Mesenchymal Transition - drug effects; Humans; IL-1β; Immunocytochemistry; Immunoreactivity; Mesenchyme; Metastases; N-Cadherin; Neoplastic Stem Cells - drug effects; Neoplastic Stem Cells - metabolism; Neoplastic Stem Cells - pathology; Pancreatic cancer; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Quercetin; Quercetin - pharmacology; Resveratrol; Resveratrol - pharmacology; Stem cells; Toxicity; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Vimentin; Vimentin - metabolism
• ISSN: 0163-5581; 1532-7914
• DOI: 10.1080/01635581.2019.1670853

Resveratrol and quercetin are phytochemicals that are found in a variety of plants. The aim of this study was to investigate the effect of resveratrol and quercetin on epithelial-mesenchymal transition (EMT) of CD133+ and CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from the PANC-1 cells by the MiniMACS system. CD133+ and CD133− PANC-1 cells were treated with different concentrations (5, 10, 25, 50, and 100 µM) of resveratrol and quercetin. Cell growth and cytotoxicity were evaluated by MTT assay. Anticancer and anti-metastatic properties of resveratrol and quercetin were determined by immunocytochemistry using antibodies (ACTA-2, IL-1β, N-cadherin, TNF-α, and vimentin). The immunostaining intensity of CD133+ cells was stronger than CD133− cells. ACTA-2, IL-1β, and N-cadherin immunoreactivities were significantly decreased, whereas TNF-α and vimentin immunoreactivities significantly increased in quercetin-treated CD133+ cells. Moreover, N-cadherin and TNF-α immunoreactivities significantly decreased in resveratrol-treated CD133+ cells. The reduction in N-cadherin and ACTA-2 immunoreactivities was higher than the increase in vimentin immunoreactivity, quercetin could prevent EMT to a greater extent than resveratrol in pancreatic cancer stem cells because of the reduced expression of N-cadherin. Quercetin could be more effective in inhibiting metastasis compared to resveratrol.