Serum from differently exercised subjects induces myogenic differentiation in LHCN-M2 human myoblasts

• Published in: Journal of sports sciences Vol. 36; no. 14; pp. 1630 - 1639
• Main Authors: Vitucci, D., Imperlini, E., Arcone, R., Alfieri, A., Canciello, A., Russomando, L., Martone, D., Cola, A., Labruna, G., Orrù, S., Tafuri, D., Mancini, A., Buono, P.
• Format: Journal Article
• Language: English
• Published: England Routledge 18.07.2018; Taylor & Francis Ltd
• Subjects: Adult; Apoptosis; Apoptosis - physiology; Autophagy - physiology; Bcl-2 protein; Cardiac Myosins - genetics; Cardiac Myosins - metabolism; Cell activation; Cell Culture Techniques; Cell Differentiation - physiology; Cell Line; Creatine; Creatine kinase; Creatine Kinase - metabolism; Culture Media; Exercise; Exercise - physiology; Gene Expression; human myoblasts; human serum; Humans; Kinases; LHCN-M2 cells; Muscle Development - physiology; Muscle Fibers, Skeletal - physiology; Muscular system; Myoblasts; Myoblasts - physiology; Myogenesis; myogenic differentiation; Myogenin; Myogenin - metabolism; myosin heavy chain-β (MyHC-β); Myosin Heavy Chains - genetics; Myosin Heavy Chains - metabolism; Myotubes; RNA, Messenger - genetics; Serum; Sport science; Young Adult; LHCN-M2 cells; myosin heavy chain-β (MyHC-β); Exercise; myogenic differentiation; human serum; human myoblasts

Myogenesis is the formation of muscle tissue from muscle precursor cells. Physical exercise induces satellite cell activation in muscle. Currently, C2C12 murine myoblast cells are used to study myogenic differentiation. Herein, we evaluated whether human LHCN-M2 myoblasts can differentiate into matu...