Contribution of Cyclopentenone Prostaglandins to the Resolution of Inflammation Through the Potentiation of Apoptosis in Activated Macrophages

• Published in: The Journal of immunology (1950) Vol. 165; no. 11; pp. 6525 - 6531
• Main Authors: Hortelano, Sonsoles, Castrillo, Antonio, Alvarez, Alberto M, Bosca, Lisardo
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.12.2000
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Apoptosis - drug effects; Apoptosis - immunology; Cell Line; Cyclopentanes - pharmacology; cyclopentenones; Drug Synergism; Inflammation Mediators - pharmacology; Macrophage Activation - drug effects; Macrophages - drug effects; Macrophages - enzymology; Macrophages - metabolism; Macrophages - pathology; MAP Kinase Signaling System - drug effects; MAP Kinase Signaling System - immunology; Mice; Microbodies - drug effects; Microbodies - metabolism; Mitogen-Activated Protein Kinases - physiology; p38 Mitogen-Activated Protein Kinases; Prostaglandin D2 - analogs & derivatives; Prostaglandin D2 - physiology; Reactive Oxygen Species - metabolism; Receptors, Cytoplasmic and Nuclear - metabolism; Superoxides - metabolism; Transcription Factors - metabolism

Activation of the macrophage cell line RAW 264.7 with LPS and IFN-gamma induces apoptosis through the synthesis of high concentrations of NO due to the expression of NO synthase-2. In addition to NO, activated macrophages release other molecules involved in the inflammatory response, such as reactiv...