A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene

• Published in: Biotechnology and bioengineering Vol. 115; no. 2; pp. 464 - 472
• Main Authors: Larroude, Macarena, Celinska, Ewelina, Back, Alexandre, Thomas, Stephan, Nicaud, Jean‐Marc, Ledesma‐Amaro, Rodrigo
• Format: Journal Article
• Language: English
• Published: United States Wiley 01.02.2018
• Subjects: beta Carotene - metabolism; Biochemistry, Molecular Biology; Biotechnology; Escherichia coli - genetics; golden gate; Life Sciences; metabolic engineering; Metabolic Networks and Pathways; Molecular biology; Molecular Networks; Promoter Regions, Genetic; promoter shuffling; synthetic biology; Synthetic Biology - methods; Yarrowia - genetics; Yarrowia - metabolism; Yarrowia lipolytica; β‐carotene; promoter shuffling; synthetic biology; β-carotene; metabolic engineering; golden gate; Yarrowia lipolytica

The increasing market demands of β‐carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. Yarrowia lipolytica, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Ac...