Rapid screening system to identify unspecific peroxygenase activity

• Published in: Clinical hemorheology and microcirculation Vol. 89; no. 4; pp. 363 - 373
• Main Authors: Schramm, Marina, Carrillo Avilés, Carlos Renato, Kalmbach, Johannes, Schmidtke, Kai-Uwe, Kiebist, Jan, Kellner, Harald, Hofrichter, Martin, Scheibner, Katrin
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.04.2025
• Subjects: Aspergillus niger - enzymology; Aspergillus niger - genetics; Clopidogrel; Mixed Function Oxygenases - metabolism; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; enzyme expression; unspecific peroxygenase; omega-3-lipids metabolism; screening assay; ABTS; drug metabolism

Unspecific peroxygenases (UPO, EC 1.11.2.1) are a valuable tool for the biocatalytic synthesis of specialty chemicals such as pharmaceutical metabolites. However, the search for new UPOs that are recombinantly expressible can be tedious and dependent on expensive equipment, especially when a large n...