Deproteinization is Necessary for the Accurate Determination of Ammonia Levels by Glutamate Dehydrogenase Assay in Blood Plasma From Subjects With Liver Injury

• Published in: Laboratory medicine Vol. 48; no. 4; pp. 339 - 345
• Main Authors: Vodenicarovova, Melita, Skalska, Hana, Holecek, Milan
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 08.11.2017
• Subjects: Ammonia; Ammonia - blood; Animals; Blood Chemical Analysis - methods; Blood Chemical Analysis - standards; Blood Proteins - isolation & purification; Dehydrogenases; Glutamate Dehydrogenase - metabolism; Humans; Linear Models; Liver Diseases - blood; Liver Diseases - metabolism; Male; NAD - metabolism; NADP - metabolism; Oxidation; Oxidation-Reduction; Rats; Rats, Wistar; Rodents; ammonia; NADH oxidation; liver enzymes; liver injury; glutamate dehydrogenase; NADPH oxidation

Abstract Objective To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)– and nicotinamide adenine dinucleotide phosphate (NADPH)–consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia. Methods We measured ammonia concen...