Systematic lipidomic analysis of yeast protein kinase and phosphatase mutants reveals novel insights into regulation of lipid homeostasis

• Published in: Molecular biology of the cell Vol. 25; no. 20; pp. 3234 - 3246
• Main Authors: da Silveira dos Santos, Aline Xavier, Riezman, Isabelle, Aguilera-Romero, Maria-Auxiliadora, David, Fabrice, Piccolis, Manuele, Loewith, Robbie, Schaad, Olivier, Riezman, Howard
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 15.10.2014
• Series: A Highlights from
• Subjects: Glycerophospholipids - analysis; Glycerophospholipids - metabolism; Homeostasis; Lipid Metabolism; Lipids - analysis; Mass Spectrometry; Mutation; Phosphoric Monoester Hydrolases - genetics; Protein Kinases - genetics; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Sphingolipids - analysis; Sphingolipids - metabolism; Sterols - analysis; Sterols - metabolism

The regulatory pathways required to maintain eukaryotic lipid homeostasis are largely unknown. We developed a systematic approach to uncover new players in the regulation of lipid homeostasis. Through an unbiased mass spectrometry–based lipidomic screening, we quantified hundreds of lipid species, i...