A nanoparticle-based method for culture-free bacterial DNA enrichment from whole blood

• Published in: Biosensors & bioelectronics Vol. 99; pp. 150 - 155
• Main Authors: Hassan, Marwa M., Ranzoni, Andrea, Cooper, Matthew A.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.01.2018
• Subjects: Bacteria; Bacterial sepsis detection; Biosensing Techniques; Blood; DNA; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Humans; Limit of Detection; Magnetic nanoparticles; Nanoparticles; POCT; Point-of-Care Systems; qPCR; Sensitivity and Specificity; Sepsis - blood; Sepsis - microbiology; Staphylococcus aureus - growth & development; Staphylococcus aureus - isolation & purification; Staphylococcus aureus - pathogenicity; qPCR; POCT; Blood; Bacterial sepsis detection; Magnetic nanoparticles

Point-of-care (POC) diagnostics are one of the quick and sensitive detection approaches used in current clinical applications, but always face a performance tradeoff between time-to-result and assay sensitivity. One critical setting where these limitations are evident is the detection of sepsis, whe...