Facile preparation of near-infrared fluorescence and magnetic resonance dual-modality imaging probes based on mesoporous organosilica nanoparticles

• Published in: Journal of colloid and interface science Vol. 539; pp. 277 - 286
• Main Authors: Li, Yanjiao, Guo, Wenwen, Su, Xiaodan, Lu, Nan, Wu, Guangyao, Ou-Yang, Lin, Dang, Meng, Tao, Jun, Teng, Zhaogang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.03.2019
• Subjects: Click-reaction; Dual-modality; Imaging probe; Mesoporous organosilica; Dual-modality; Click-reaction; Imaging probe; Mesoporous organosilica
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2018.12.067

Tumor targeting near-infrared fluorescence and magnetic resonance dual-modality imaging probes are prepared by connecting functional molecules on thioether-bridged mesoporous organosilica nanoparticles via click reaction. [Display omitted] In this work, near-infrared fluorescence (NIRF) and magnetic resonance (MR) dual-modality imaging probes are prepared by conjugating maleimide derivative cyanine dye (Mal-Cy5.5), gadolinium‐diethylenetriamine pentaacetic acid (Gd-DTPA), and RGD peptide (Mal-PEG2-RGD) on thioether-bridged mesoporous organosilica nanoparticles (MONs) via click reaction. Fourier transform infrared (FT-IR) spectra, zeta potentials, UV–vis spectra, and energy dispersive X-ray (EDX) spectrum confirm the successful modifications of the functional molecules on the MONs. The prepared MON-Gd-Cy5.5-RGD probes shows excellent NIRF and MR imaging properties, and the relaxivity rate (r1) is measured up to 2.85 mM−1 s −1. In addition, the MON-Gd-Cy5.5-RGD probes show excellent in vitro and in vivo biocompatibility. Confocal laser scanning microscopy and flow cytometry demonstrate that the MON-Gd-Cy5.5-RGD can efficiently target to MDA-MB-231 tumor cells. Additionally, ex vivo NIFR and in vivo MR imaging demonstrate that the MON-Gd-Cy5.5-RGD probes can accumulate in tumor and improve the signals of tumor.