High-throughput, single-microbe genomics with strain resolution, applied to a human gut microbiome - Zheng, Wenshan; Zhao, Shijie; Yin, Yehang; Zhang, Huidan; Needham, David M.; Evans, Ethan D.; Dai, Chengzhen L.; Lu, Peter J.; Alm, Eric J.; Weitz, David A. (Science (American Association for the Advancement of Science), 2022)
Keywords: Amplification; Antibiotic resistance; Antibiotics; Bacteria; Bacteria - genetics; Bacteriophages - genetics; Bacteroides - genetics; Bacteroides - virology; Bar codes; BASIC BIOLOGICAL SCIENCES; Biological research; Cell culture; Community Relations; Computer applications; Deoxyribonucleic acid; Digestive system; DNA; DNA, Bacterial - genetics; Droplets; Encapsulation; Feces; Gastrointestinal Microbiome - genetics; Gene transfer; Gene Transfer, Horizontal; Genome, Bacterial; Genomes; Genomics; Genomics - methods; Gut microbiota; High-Throughput Nucleotide Sequencing; Horizontal transfer; Human populations; Humans; Intestinal microflora; Metagenomics; Microbial activity; Microbial Interactions; Microbiomes; Microbiota; Microfluidics; Microorganisms; Nucleotide sequence; Phages; Single-Cell Analysis - methods; Species; Strain; Strains (organisms); Workflow