Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica

• Published in: Journal of biotechnology Vol. 397; pp. 32 - 43
• Main Authors: Briel, Simon Carl-Philipp, Feuser, Nicolas, Moldenhauer, Eva Johanna, Kabisch, Johannes, Neubauer, Peter, Junne, Stefan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2025
• Subjects: biotechnology; fatty acid composition; Fatty Acids - analysis; Fatty Acids - metabolism; Fed-batch; flow cytometry; Food production; gas chromatography; Holography - methods; Lipid Metabolism; Lipids - analysis; Long-chain fatty acids; microscopy; Microscopy - methods; nutritive value; Oleaginous yeast; Process analytical technology; process control; Quantitative Phase Imaging; refractive index; Single-Cell Analysis - methods; Yarrowia - cytology; Yarrowia - metabolism; Yarrowia lipolytica; yeasts; Process analytical technology; Food production; Fed-batch; Oleaginous yeast; Long-chain fatty acids

Digital holographic microscopy (DHM) is a label-free analytical technique for the determination of the cells’ volume and their cytosolic refractive index. Here, we demonstrate the suitability of DHM for the quantification of total lipid accumulation in the oleaginous yeast Yarrowia lipolytica. Prese...