Proteomic profiling of tear fluid as a promising non-invasive screening test for colon cancer

Current screening options for colorectal cancer (CRC) are either invasive (colonoscopy) or have lower sensitivity to identify pre-malignant lesions (fecal immunochemical test). We proposed to identify protein profiles in tears of patients with both pre-malignant polyps and CRC; these profiles could...

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Published inThe American journal of surgery Vol. 224; no. 1; pp. 19 - 24
Main Authors Kaufmann, Yihong, Byrum, Stephanie D., Acott, Alison A., Siegel, Eric R., Washam, Charity L., Klimberg, V. Suzanne, Mancino, Anne T.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.2022
Elsevier Limited
Subjects
Antigens
Biomarker
Biomarkers
Colon
Colon cancer
Colonoscopy
Colorectal cancer
Colorectal carcinoma
Disease
Endoscopy
Fluids
Gene expression
Lesions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metastasis
Peptides
Polyps
Protein interaction
Proteins
Proteomics
Risk
Scientific imaging
Screening
Spectroscopy
Tear fluid
Tumors
Tear fluid
Screening
Biomarker
Colon cancer
Proteomics
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Summary:Current screening options for colorectal cancer (CRC) are either invasive (colonoscopy) or have lower sensitivity to identify pre-malignant lesions (fecal immunochemical test). We proposed to identify protein profiles in tears of patients with both pre-malignant polyps and CRC; these profiles could have potential as a noninvasive screening test. Colonoscopy patients were divided into “high risk” group (CRC and tubular adenomatous polyp) and “low risk” (normal and hyperplastic polyps). Tear fluids from patients were analyzed by Liquid Chromatography Mass Spectrometry/Mass Spectrometry. The data were analyzed for protein expression, protein-protein interaction and gene set enrichment. The results showed 80 proteins (18 up-regulated and 62 down-regulated) significantly differentiated in “high-risk” compared to “low-risk”; Twenty-eight of these show protein-protein interactions, 9 of which were associated with pathways demonstrated to be altered in CRC patients. Our pilot data, though limited, demonstrated tear protein profiling could distinguish the groups of patients with and without colon lesions. •Proteomic markers in tears.•Proteins distinguish polyp/CRC vs benign.•Potential screening tool.
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ISSN:0002-9610
1879-1883
DOI:10.1016/j.amjsurg.2022.03.029