Secretion of 2,3-dihydroxyisovalerate as a limiting factor for isobutanol production in Saccharomyces cerevisiae

• Published in: FEMS yeast research Vol. 17; no. 3
• Main Authors: Generoso, Wesley Cardoso, Brinek, Martin, Dietz, Heiko, Oreb, Mislav, Boles, Eckhard
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.05.2017
• Subjects: Biofuels; Butanols - metabolism; Butyrates - antagonists & inhibitors; Butyrates - metabolism; Clonal deletion; Deletion mutant; Ethanol; Fermentation; Gene Deletion; Genes, Fungal; Genetic engineering; Genetic Engineering - methods; Industrial Microbiology; Intermediates; Kinetics; Lactic acid; Microarray Analysis; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Yeast; Ehrlich pathway; xylose fermentation; metabolite secretion; Ethanol Red; branched-chain amino acid pathway; isobutanol

Isobutanol is a superior biofuel compared to ethanol, and it is naturally produced by yeasts. Previously, Saccharomyces cerevisiae has been genetically engineered to improve isobutanol production. We found that yeast cells engineered for a cytosolic isobutanol biosynthesis secrete large amounts of t...