Biochemical Similarity of Expressed Human Prorenin and Native Inactive Renin

• Published in: Hypertension (Dallas, Tex. 1979) Vol. 8; no. 6, Part 2 Suppl II; pp. II-78 - II-83
• Main Authors: HSUEH, WILLA A, DO, YUNG SHUN, SHINAGAWA, TATSUO, TAM, HELEN, PONTE, PHYLLIS A, BAXTER, JOHN D, SHINE, JOHN, FRITZ, LAWRENCE C
• Format: Journal Article
• Language: English
• Published: United States American Heart Association, Inc 01.06.1986
• Subjects: Cold Temperature; Cross Reactions; Electrophoresis, Polyacrylamide Gel; Enzyme Precursors - analysis; Enzyme Precursors - immunology; Humans; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Renin - analysis; Renin - biosynthesis; Renin - immunology; Triazines; Trypsin - pharmacology

Prorenin is secreted by mammalian cells transfected with a human preprorenin expression construct. The purpose of this investigation was to compare the physicochemical properties of expressed prorenin in culture medium with the known characteristics of human inactive renin, which accounts for nearly...