Primer set evaluation and sampling method assessment for the monitoring of fish communities in the North‐western part of the Mediterranean Sea through eDNA metabarcoding

• Published in: Environmental DNA (Hoboken, N.J.) Vol. 6; no. 3
• Main Authors: Roblet, Sylvain, Priouzeau, Fabrice, Gambini, Gilles, Dérijard, Benoit, Sabourault, Cécile
• Format: Journal Article
• Language: English
• Published: Hoboken John Wiley & Sons, Inc 01.05.2024; Wiley
• Subjects: Bar codes; biodiversity assessment; Commercial fishing; Cytochrome; DNA barcoding; eDNA metabarcoding; Efficiency; Environmental DNA; Environmental monitoring; Filters; Fish; fish monitoring; Fishing; North‐western Mediterranean Sea; PCR primer sets; Pilot projects; Pore size; Porosity; rRNA 12S; rRNA 16S; Sampling; Sampling methods; sampling strategy; Species composition; Surface water; Taxonomy; Water purification; Mediterranean Sea

Environmental DNA (eDNA) metabarcoding appears to be a promising tool to survey fish communities. However, the effectiveness of this method relies on primer set performance and on a robust sampling strategy. While some studies have evaluated the efficiency of several primers for fish detection, it h...