Effect of Trehalose and Ceftriaxone on the Stability of Aggregating-Prone Tau Peptide Containing PHF6 Sequence: An SRCD Study

• Published in: International journal of molecular sciences Vol. 23; no. 6; p. 2932
• Main Authors: Honisch, Claudia, Torni, Federica, Hussain, Rohanah, Ruzza, Paolo, Siligardi, Giuliano
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.03.2022; MDPI
• Subjects: Algae; Alzheimer Disease - metabolism; Alzheimer's disease; Antibiotics; Ceftriaxone; Ceftriaxone - pharmacology; Circular Dichroism; Cytoskeleton; Decomposition; Diamonds; Dichroism; Filaments; Food processing; Heparin; Humans; intrinsically disordered proteins; Kinetics; Microtubules; Neurofibrillary tangles; Neurofibrillary Tangles - metabolism; Peptides; Peptides - chemistry; Phase transitions; protein aggregation; Protein folding; Proteins; Repressor Proteins - metabolism; Spectrum analysis; Stability; Synchrotron radiation; Synchrotrons; Tau protein; tau Proteins - metabolism; tauopathies; Trehalose; Trehalose - metabolism; Trehalose - pharmacology; ceftriaxone; synchrotron radiation circular dichroism; protein aggregation; conformational stability; tauopathies; trehalose; transmission electron microscopy; intrinsically disordered proteins; tau protein
• DOI: 10.3390/ijms23062932
• ISSN: 1422-0067; 1661-6596

The tau protein, a soluble protein associated with microtubules, which is involved in the assembly and stabilization of cytoskeletal elements, was found to form neurofibrillary tangles in different neurodegenerative diseases. Insoluble tau aggregates were observed to be organized in paired helical filaments (PHFs) and straight filaments (SFs). Recently, two small sequences (306-311 and 275-280) in the microtubule-binding region (MTBR), named PHF6 and PHF6*, respectively, were found to be essential for tau aggregation. Since a possible therapeutic approach consists of impairing amyloid formation either by stabilizing the native proteins or reducing the level of amyloid precursors, here we use synchrotron radiation circular dichroism (SRCD) at Diamond B23 beamline to evaluate the inhibitory effects of two small molecules, trehalose and ceftriaxone, against the aggregation of a small peptide containing the PHF6* sequence. Our results indicate that both these molecules, ceftriaxone and trehalose, increased the stability of the peptide toward aggregation, in particular that induced by heparin. With trehalose being present in many fruits, vegetables, algae and processed foods, these results support the need to investigate whether a diet richer in trehalose might exert a protective effect toward pathologies linked to protein misfolding.