A method for the extraction of high quality fungal RNA suitable for RNA-seq

• Published in: Journal of microbiological methods Vol. 170; p. 105855
• Main Authors: Cortés-Maldonado, Leyda, Marcial-Quino, Jaime, Gómez-Manzo, Saúl, Fierro, Francisco, Tomasini, Araceli
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2020
• Subjects: Amylomyces rouxii; cDNA libraries; complementary DNA; deoxyribonuclease I; enzymatic hydrolysis; enzyme activity; Filamentous fungi; Fungal RNA purification; fungi; Gene Expression Profiling - methods; Mucorales - genetics; Mucorales - isolation & purification; purification methods; ribonucleases; RNA; RNA, Fungal - chemistry; RNA, Fungal - isolation & purification; RNA-seq; RNA-Seq - methods; salts; sequence analysis; transcriptomics; 18-30G; RNA-seq; Amylomyces rouxii; Filamentous fungi; RIN; TAE; cDNA; NGS; PCP; RT-qPCR; PCR; Fungal RNA purification

Transcriptomic analysis is an OMICs technology that is becoming indispensable to understand and get a complete picture of cell functioning and adaptation to the environmental cues the cell is continuously receiving. Among the techniques available to perform transcriptomics, RNA-seq is becoming the m...