Epitopes for chicken monoclonal antibodies in spleens of selected Japanese quail lines

• Published in: Developmental and comparative immunology Vol. 23; no. 6; pp. 501 - 509
• Main Authors: Lovitt, Heather L, Siegel, Herbert S, Weinstock, Daniel, Marks, Henry L
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.09.1999
• Subjects: Animals; Antibodies, Monoclonal - metabolism; Atherosclerosis; Brucella abortus - immunology; CD-8; CD8 Antigens - immunology; CD8 Antigens - metabolism; Chicken monoclonal antibodies; Chickens; cholesterol; Coturnix - immunology; Coturnix japonica; Cross Reactions; Epitopes - immunology; Epitopes - metabolism; Erythrocytes - immunology; Female; Japanese quail; Lymphocyte epitopes; Male; Mice; Plasma cholesterol; Receptors, Antigen, T-Cell - immunology; Receptors, Antigen, T-Cell - metabolism; Sheep; Spleen - cytology; Spleen - immunology; TCR-3; TCR, T-cell receptor; c-mAb, chicken monoclonal antibodies; Ab, antibody; TI, T-cell independent; Chicken monoclonal antibodies; Plasma cholesterol; Japanese quail; Ag, antigens; Bu-1a/b, B-cell marker; CD-8; ACTH, adrenocorticotropin; TD, T-cell dependent; CD, cluster of differentiation; TCR-3; Atherosclerosis; Lymphocyte epitopes
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/S0145-305X(99)00033-6

A line of Japanese quail selected for high plasma cholesterol is highly susceptible to diet-induced atherosclerosis. Lymphocyte epitopes recognized by mouse anti-chicken monoclonal antibodies (c-mAb), TCR-1, TCR-2, TCR-3, CD-3, CD-4, CD-8, and BU-1a/b were reacted with spleens from quail selected for high (HL) and low (LL) plasma total cholesterol and their nonselected controls (CL). Cross reactivity to c-mAb and effect of line and gender were immunohistochemically evaluated. Chicken spleens were positive controls. Quail were immunologically stimulated with either sheep red blood cells (SRBC) or Brucella abortus 2 weeks before spleens were removed. Quail spleen epitopes of all lines recognized TCR-3 and CD-8 c-mAb, but no other c-mAb. Number of reacting cells and staining intensity to the TCR-3 c-mAb were greater in the HL than in the LL regardless of the stimulating Ag or dose used. For the CD-8 c-mAb, there were no differences among lines in birds receiving SRBC. In B. abortus-immunized birds, sex×line interactions indicated that males of the HL and CL had lower responses than females but LL males were not different than females. TCR-3 and CD8 c-mAb may be useful in studying immunological mechanisms for atherosclerosis in Japanese quail.