ROS/TNF-α Crosstalk Triggers the Expression of IL-8 and MCP-1 in Human Monocytic THP-1 Cells via the NF-κB and ERK1/2 Mediated Signaling

IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 a...

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Published inInternational journal of molecular sciences Vol. 22; no. 19; p. 10519
Main Authors Akhter, Nadeem, Wilson, Ajit, Thomas, Reeby, Al-Rashed, Fatema, Kochumon, Shihab, Al-Roub, Areej, Arefanian, Hossein, Al-Madhoun, Ashraf, Al-Mulla, Fahd, Ahmad, Rasheed, Sindhu, Sardar
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.10.2021
MDPI
Subjects
Body mass index
Chemokines
Cytokines
Fibroblasts
Flow cytometry
Gene expression
Hypoxia
Inflammation
Insulin resistance
Kinases
Metabolic disorders
Metabolic syndrome
Microscopy
Neutrophils
Obesity
Overweight
Oxidative stress
Protein expression
Proteins
Reactive oxygen species
Tumor necrosis factor-TNF
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Abstract IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 adipose expression was detected in lean, overweight, and obese individuals, 15 each, using immunohistochemistry. To detect the role of reactive oxygen species (ROS)/TNF-α synergy as a chemokine driver, THP-1 cells were stimulated with TNF-α, with/without H2O2 or hypoxia. Target gene expression was measured by qRT-PCR, proteins by flow cytometry/confocal microscopy, ROS by DCFH-DA assay, and signaling pathways by immunoblotting. IL-8/MCP-1 adipose expression was significantly higher in obese/overweight. Furthermore, IL-8/MCP-1 mRNA/protein was amplified in monocytic cells following stimulation with TNF-α in the presence of H2O2 or hypoxia (p ˂ 0.0001). Synergistic chemokine upregulation was related to the ROS levels, while pre-treatments with NAC suppressed this chemokine elevation (p ≤ 0.01). The ROS/TNF-α crosstalk involved upregulation of CHOP, ERN1, HIF1A, and NF-κB/ERK-1,2 mediated signaling. In conclusion, IL-8/MCP-1 adipose expression is elevated in obesity. Mechanistically, ROS/TNF-α crosstalk may drive expression of these chemokines in monocytic cells by inducing ER stress, HIF1A stabilization, and signaling via NF-κB/ERK-1,2. NAC had inhibitory effect on oxidative stress-driven IL-8/MCP-1 expression, which may have therapeutic significance regarding meta-inflammation.
AbstractList IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 adipose expression was detected in lean, overweight, and obese individuals, 15 each, using immunohistochemistry. To detect the role of reactive oxygen species (ROS)/TNF-α synergy as a chemokine driver, THP-1 cells were stimulated with TNF-α, with/without H 2 O 2 or hypoxia. Target gene expression was measured by qRT-PCR, proteins by flow cytometry/confocal microscopy, ROS by DCFH-DA assay, and signaling pathways by immunoblotting. IL-8/MCP-1 adipose expression was significantly higher in obese/overweight. Furthermore, IL-8/MCP-1 mRNA/protein was amplified in monocytic cells following stimulation with TNF-α in the presence of H 2 O 2 or hypoxia ( p ˂ 0.0001). Synergistic chemokine upregulation was related to the ROS levels, while pre-treatments with NAC suppressed this chemokine elevation ( p ≤ 0.01). The ROS/TNF-α crosstalk involved upregulation of CHOP , ERN1 , HIF1A , and NF-κB/ERK-1,2 mediated signaling. In conclusion, IL-8/MCP-1 adipose expression is elevated in obesity. Mechanistically, ROS/TNF-α crosstalk may drive expression of these chemokines in monocytic cells by inducing ER stress, HIF1A stabilization, and signaling via NF-κB/ERK-1,2. NAC had inhibitory effect on oxidative stress-driven IL-8/MCP-1 expression, which may have therapeutic significance regarding meta-inflammation.
IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 adipose expression was detected in lean, overweight, and obese individuals, 15 each, using immunohistochemistry. To detect the role of reactive oxygen species (ROS)/TNF-α synergy as a chemokine driver, THP-1 cells were stimulated with TNF-α, with/without H2O2 or hypoxia. Target gene expression was measured by qRT-PCR, proteins by flow cytometry/confocal microscopy, ROS by DCFH-DA assay, and signaling pathways by immunoblotting. IL-8/MCP-1 adipose expression was significantly higher in obese/overweight. Furthermore, IL-8/MCP-1 mRNA/protein was amplified in monocytic cells following stimulation with TNF-α in the presence of H2O2 or hypoxia (p ˂ 0.0001). Synergistic chemokine upregulation was related to the ROS levels, while pre-treatments with NAC suppressed this chemokine elevation (p ≤ 0.01). The ROS/TNF-α crosstalk involved upregulation of CHOP, ERN1, HIF1A, and NF-κB/ERK-1,2 mediated signaling. In conclusion, IL-8/MCP-1 adipose expression is elevated in obesity. Mechanistically, ROS/TNF-α crosstalk may drive expression of these chemokines in monocytic cells by inducing ER stress, HIF1A stabilization, and signaling via NF-κB/ERK-1,2. NAC had inhibitory effect on oxidative stress-driven IL-8/MCP-1 expression, which may have therapeutic significance regarding meta-inflammation.
IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 adipose expression was detected in lean, overweight, and obese individuals, 15 each, using immunohistochemistry. To detect the role of reactive oxygen species (ROS)/TNF-α synergy as a chemokine driver, THP-1 cells were stimulated with TNF-α, with/without H2O2 or hypoxia. Target gene expression was measured by qRT-PCR, proteins by flow cytometry/confocal microscopy, ROS by DCFH-DA assay, and signaling pathways by immunoblotting. IL-8/MCP-1 adipose expression was significantly higher in obese/overweight. Furthermore, IL-8/MCP-1 mRNA/protein was amplified in monocytic cells following stimulation with TNF-α in the presence of H2O2 or hypoxia (p ˂ 0.0001). Synergistic chemokine upregulation was related to the ROS levels, while pre-treatments with NAC suppressed this chemokine elevation (p ≤ 0.01). The ROS/TNF-α crosstalk involved upregulation of CHOP, ERN1, HIF1A, and NF-κB/ERK-1,2 mediated signaling. In conclusion, IL-8/MCP-1 adipose expression is elevated in obesity. Mechanistically, ROS/TNF-α crosstalk may drive expression of these chemokines in monocytic cells by inducing ER stress, HIF1A stabilization, and signaling via NF-κB/ERK-1,2. NAC had inhibitory effect on oxidative stress-driven IL-8/MCP-1 expression, which may have therapeutic significance regarding meta-inflammation.IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it remains unclear whether the TNF-α/oxidative stress interplay can trigger IL-8/MCP-1 expression and, if so, by which mechanism(s). IL-8/MCP-1 adipose expression was detected in lean, overweight, and obese individuals, 15 each, using immunohistochemistry. To detect the role of reactive oxygen species (ROS)/TNF-α synergy as a chemokine driver, THP-1 cells were stimulated with TNF-α, with/without H2O2 or hypoxia. Target gene expression was measured by qRT-PCR, proteins by flow cytometry/confocal microscopy, ROS by DCFH-DA assay, and signaling pathways by immunoblotting. IL-8/MCP-1 adipose expression was significantly higher in obese/overweight. Furthermore, IL-8/MCP-1 mRNA/protein was amplified in monocytic cells following stimulation with TNF-α in the presence of H2O2 or hypoxia (p ˂ 0.0001). Synergistic chemokine upregulation was related to the ROS levels, while pre-treatments with NAC suppressed this chemokine elevation (p ≤ 0.01). The ROS/TNF-α crosstalk involved upregulation of CHOP, ERN1, HIF1A, and NF-κB/ERK-1,2 mediated signaling. In conclusion, IL-8/MCP-1 adipose expression is elevated in obesity. Mechanistically, ROS/TNF-α crosstalk may drive expression of these chemokines in monocytic cells by inducing ER stress, HIF1A stabilization, and signaling via NF-κB/ERK-1,2. NAC had inhibitory effect on oxidative stress-driven IL-8/MCP-1 expression, which may have therapeutic significance regarding meta-inflammation.
Author Al-Roub, Areej
Al-Madhoun, Ashraf
Arefanian, Hossein
Sindhu, Sardar
Al-Rashed, Fatema
Ahmad, Rasheed
Thomas, Reeby
Akhter, Nadeem
Wilson, Ajit
Al-Mulla, Fahd
Kochumon, Shihab
AuthorAffiliation 1 Department of Immunology & Microbiology, Dasman Diabetes Institute, P.O. Box 1180, Dasman 15462, Kuwait; nadeem.akhter@dasmaninstitute.org (N.A.); ajit.wilson@dasmaninstitute.org (A.W.); reeby.thomas@dasmaninstitute.org (R.T.); fatema.alrashed@dasmaninstitute.org (F.A.-R.); shihab.kochumon@dasmaninstitute.org (S.K.); areej.abualroub@dasmaninstitute.org (A.A.-R.); hossein.arefanian@dasmaninstitute.org (H.A.); rasheed.ahmad@dasmaninstitute.org (R.A.)
2 Department of Genetics & Bioinformatics, Dasman Diabetes Institute, P.O. Box 1180, Dasman 15462, Kuwait; ashraf.madhoun@dasmaninstitute.org (A.A.-M.); fahd.almulla@dasmaninstitute.org (F.A.-M.)
3 Animal & Imaging Core Facility, Dasman Diabetes Institute, P.O. Box 1180, Dasman 15462, Kuwait
AuthorAffiliation_xml – name: 1 Department of Immunology & Microbiology, Dasman Diabetes Institute, P.O. Box 1180, Dasman 15462, Kuwait; nadeem.akhter@dasmaninstitute.org (N.A.); ajit.wilson@dasmaninstitute.org (A.W.); reeby.thomas@dasmaninstitute.org (R.T.); fatema.alrashed@dasmaninstitute.org (F.A.-R.); shihab.kochumon@dasmaninstitute.org (S.K.); areej.abualroub@dasmaninstitute.org (A.A.-R.); hossein.arefanian@dasmaninstitute.org (H.A.); rasheed.ahmad@dasmaninstitute.org (R.A.)
– name: 3 Animal & Imaging Core Facility, Dasman Diabetes Institute, P.O. Box 1180, Dasman 15462, Kuwait
– name: 2 Department of Genetics & Bioinformatics, Dasman Diabetes Institute, P.O. Box 1180, Dasman 15462, Kuwait; ashraf.madhoun@dasmaninstitute.org (A.A.-M.); fahd.almulla@dasmaninstitute.org (F.A.-M.)
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Cites_doi 10.1210/jc.2002-021733
10.1128/MCB.00060-08
10.1007/s00424-006-0055-8
10.1152/ajpheart.00716.2003
10.1371/journal.pone.0136755
10.1007/BF02708432
10.1146/annurev-immunol-032414-112116
10.1186/1475-2840-2-5
10.1155/2012/504952
10.1155/2014/181450
10.2337/diab.43.11.1271
10.1038/sj.ijo.0803177
10.1016/j.bbamem.2012.08.013
10.3858/emm.2008.40.4.461
10.1159/000478869
10.1016/j.biocel.2006.07.001
10.3390/ijms12053117
10.1073/pnas.87.13.5134
10.1007/s00592-017-1039-1
10.1161/01.ATV.0000258979.92828.bc
10.1126/science.7678183
10.1073/pnas.1621188114
10.1152/ajprenal.00111.2015
10.3390/ijms11103769
10.1016/j.biocel.2006.03.016
10.2174/1381612043452866
10.1038/nri722
10.1101/533018
10.1189/jlb.0307195
10.1016/j.bbamem.2006.02.015
10.1038/nri.2016.62
10.1016/j.it.2004.09.015
10.1007/BF03165759
10.1161/01.RES.0000115557.25127.8D
10.1128/IAI.67.2.688-693.1999
10.1016/j.trim.2016.02.003
10.1615/CritRevEukarGeneExpr.v20.i2.10
10.1007/s11695-008-9424-z
10.1016/S0891-5849(00)00252-5
10.1186/s11658-018-0077-1
10.1002/eji.1830270508
10.1017/S0029665100000732
10.1055/s-2007-978682
10.1073/pnas.0907705107
10.1007/s10571-014-0069-2
10.1038/ki.1995.410
10.1038/sj.ijo.0803259
10.1016/0165-5728(94)90220-8
10.1038/39335
10.1016/j.ejcdt.2013.02.012
10.4049/jimmunol.139.3.788
10.1002/1097-0029(20000801)50:3<184::AID-JEMT2>3.0.CO;2-H
10.1007/s11325-015-1252-5
10.1089/ars.2008.2140
10.1093/molehr/7.8.787
10.1152/ajpcell.00558.2004
10.1016/S0021-9258(19)61441-9
10.1074/jbc.M103074200
10.1186/1742-2094-4-12
10.4103/0971-5916.93435
10.1165/rcmb.2007-0197OC
10.1210/jc.2002-020135
10.1007/s11064-018-2479-x
10.1155/2012/943706
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PublicationDate_xml – month: 10
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  text: 2021-10-01
  day: 01
PublicationDecade 2020
PublicationPlace Basel
PublicationPlace_xml – name: Basel
PublicationTitle International journal of molecular sciences
PublicationYear 2021
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Bruun (ref_8) 2000; 32
Mourad (ref_46) 2013; 62
Wang (ref_60) 2006; 38
Qutub (ref_26) 2008; 28
Rovin (ref_66) 1995; 48
Martin (ref_67) 1997; 27
Mantovani (ref_3) 2004; 25
ref_55
Proudfoot (ref_2) 2002; 2
Elliott (ref_63) 2001; 7
Hotamisligil (ref_7) 1994; 43
Baran (ref_38) 2004; 10
Barna (ref_11) 1994; 50
Naziroglu (ref_43) 2014; 34
Grootjans (ref_53) 2016; 16
Catalan (ref_6) 2007; 17
Bruun (ref_33) 2001; 86
Singh (ref_44) 2020; 10
Palacio (ref_22) 2013; 1828
Jain (ref_49) 2009; 11
Yao (ref_1) 2014; 2014
Vincent (ref_17) 2006; 30
Chuang (ref_62) 2016; 20
Martinez (ref_19) 2006; 62
Kim (ref_42) 2008; 40
Khanjani (ref_65) 2012; 2012
Marie (ref_61) 1999; 67
ref_23
Straczkowski (ref_4) 2002; 87
Kim (ref_14) 2006; 30
Bienert (ref_39) 2006; 1758
Baldassari (ref_21) 2013; 2013
Straczkowski (ref_16) 2003; 2
Tufanli (ref_54) 2017; 114
Kim (ref_32) 2010; 11
Bonello (ref_24) 2007; 27
Horak (ref_25) 2010; 107
Hotamisligil (ref_28) 1993; 259
Kodama (ref_52) 2005; 289
(ref_68) 2000; 59
Qin (ref_48) 2018; 43
Parameswaran (ref_13) 2010; 20
Anand (ref_57) 2007; 82
Callaghan (ref_59) 2007; 4
Kunsch (ref_64) 1993; 13
Stenlof (ref_41) 2003; 88
Kumar (ref_47) 2016; 35
Chen (ref_34) 2004; 286
Hensley (ref_35) 2000; 28
Balasubramanyam (ref_45) 2003; 28
Richard (ref_56) 2000; 275
Yoshimura (ref_9) 1987; 139
Cushing (ref_10) 1990; 87
Bautista (ref_18) 2011; 12
Swaroop (ref_15) 2012; 135
Cimini (ref_5) 2017; 54
Feng (ref_58) 2018; 23
Galassetti (ref_27) 2012; 2012
Idriss (ref_12) 2000; 50
Sverrisson (ref_31) 2015; 309
Chandel (ref_36) 2001; 276
Bettigole (ref_50) 2015; 33
Wassmann (ref_20) 2004; 94
Wang (ref_30) 2017; 42
Valko (ref_40) 2007; 39
Vij (ref_51) 2008; 38
Uysal (ref_29) 1997; 389
Wang (ref_37) 2006; 452
References_xml – volume: 88
  start-page: 4379
  year: 2003
  ident: ref_41
  article-title: Interleukin-6 levels in the central nervous system are negatively correlated with fat mass in overweight/obese subjects
  publication-title: J. Clin. Endocrinol. Metab.
  doi: 10.1210/jc.2002-021733
– volume: 28
  start-page: 5106
  year: 2008
  ident: ref_26
  article-title: Reactive Oxygen Species Regulate Hypoxia-Inducible Factor 1α Differentially in Cancer and Ischemia
  publication-title: Mol. Cell. Biol.
  doi: 10.1128/MCB.00060-08
– volume: 452
  start-page: 418
  year: 2006
  ident: ref_37
  article-title: Acute and prolonged effects of TNF-alpha on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture
  publication-title: Pflugers Arch.
  doi: 10.1007/s00424-006-0055-8
– volume: 286
  start-page: H1001
  year: 2004
  ident: ref_34
  article-title: Superoxide, H2O2, and iron are required for TNF-α-induced MCP-1 gene expression in endothelial cells: Role of Rac1 and NADPH oxidase
  publication-title: Am. J. Physiol. Heart Circ. Physiol.
  doi: 10.1152/ajpheart.00716.2003
– ident: ref_23
  doi: 10.1371/journal.pone.0136755
– volume: 28
  start-page: 715
  year: 2003
  ident: ref_45
  article-title: Curcumin-induced inhibition of cellular reactive oxygen species generation: Novel therapeutic implications
  publication-title: J. Biosci.
  doi: 10.1007/BF02708432
– volume: 33
  start-page: 107
  year: 2015
  ident: ref_50
  article-title: Endoplasmic reticulum stress in immunity
  publication-title: Annu. Rev. Immunol.
  doi: 10.1146/annurev-immunol-032414-112116
– volume: 2
  start-page: 5
  year: 2003
  ident: ref_16
  article-title: Plasma interleukin 8 concentrations in obese subjects with impaired glucose tolerance
  publication-title: Cardiovasc. Diabetol.
  doi: 10.1186/1475-2840-2-5
– volume: 2012
  start-page: 504952
  year: 2012
  ident: ref_65
  article-title: NFkappaB and AP-1 drive human myometrial IL8 expression
  publication-title: Mediators Inflamm.
  doi: 10.1155/2012/504952
– volume: 2014
  start-page: 181450
  year: 2014
  ident: ref_1
  article-title: Roles of the chemokine system in development of obesity, insulin resistance, and cardiovascular disease
  publication-title: J. Immunol. Res.
  doi: 10.1155/2014/181450
– volume: 43
  start-page: 1271
  year: 1994
  ident: ref_7
  article-title: Tumor Necrosis Factor α: A Key Component of the Obesity-Diabetes Link
  publication-title: Diabetes
  doi: 10.2337/diab.43.11.1271
– volume: 30
  start-page: 400
  year: 2006
  ident: ref_17
  article-title: Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans
  publication-title: Int. J. Obes.
  doi: 10.1038/sj.ijo.0803177
– volume: 1828
  start-page: 357
  year: 2013
  ident: ref_22
  article-title: Effect of oxidative stress on plasma membrane fluidity of THP-1 induced macrophages
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbamem.2012.08.013
– volume: 40
  start-page: 461
  year: 2008
  ident: ref_42
  article-title: Regulation of pro-inflammatory responses by lipoxygenases via intracellular reactive oxygen species in vitro and in vivo
  publication-title: Exp. Mol. Med.
  doi: 10.3858/emm.2008.40.4.461
– volume: 42
  start-page: 416
  year: 2017
  ident: ref_30
  article-title: TNF-alpha Deficiency Prevents Renal Inflammation and Oxidative Stress in Obese Mice
  publication-title: Kidney Blood Press. Res.
  doi: 10.1159/000478869
– volume: 39
  start-page: 44
  year: 2007
  ident: ref_40
  article-title: Free radicals and antioxidants in normal physiological functions and human disease
  publication-title: Int. J. Biochem. Cell. Biol.
  doi: 10.1016/j.biocel.2006.07.001
– volume: 12
  start-page: 3117
  year: 2011
  ident: ref_18
  article-title: Inflammation, oxidative stress, and obesity
  publication-title: Int. J. Mol. Sci.
  doi: 10.3390/ijms12053117
– volume: 13
  start-page: 6137
  year: 1993
  ident: ref_64
  article-title: NF-kappa B subunit-specific regulation of the interleukin-8 promoter
  publication-title: Mol. Cell. Biol.
– volume: 87
  start-page: 5134
  year: 1990
  ident: ref_10
  article-title: Minimally modified low density lipoprotein induces monocyte chemotactic protein 1 in human endothelial cells and smooth muscle cells
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.87.13.5134
– volume: 54
  start-page: 961
  year: 2017
  ident: ref_5
  article-title: Circulating IL-8 levels are increased in patients with type 2 diabetes and associated with worse inflammatory and cardiometabolic profile
  publication-title: Acta Diabetol.
  doi: 10.1007/s00592-017-1039-1
– volume: 27
  start-page: 755
  year: 2007
  ident: ref_24
  article-title: Reactive oxygen species activate the HIF-1alpha promoter via a functional NFkappaB site
  publication-title: Arterioscler. Thromb. Vasc. Biol.
  doi: 10.1161/01.ATV.0000258979.92828.bc
– volume: 10
  start-page: 484
  year: 2020
  ident: ref_44
  article-title: Curcumin has Protective Effects on ROS Production and Redox Imbalance in an Experimental Oxidative-Stressed Model of Rat
  publication-title: J. Biol. Active Prod. Nat.
– volume: 259
  start-page: 87
  year: 1993
  ident: ref_28
  article-title: Adipose expression of tumor necrosis factor-alpha: Direct role in obesity-linked insulin resistance
  publication-title: Science
  doi: 10.1126/science.7678183
– volume: 114
  start-page: E1395
  year: 2017
  ident: ref_54
  article-title: Targeting IRE1 with small molecules counteracts progression of atherosclerosis
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1621188114
– volume: 309
  start-page: F800
  year: 2015
  ident: ref_31
  article-title: Acute reactive oxygen species (ROS)-dependent effects of IL-1β, TNF-α, and IL-6 on the glomerular filtration barrier (GFB) in vivo
  publication-title: Am. J. Physiol. Renal Physiol.
  doi: 10.1152/ajprenal.00111.2015
– volume: 11
  start-page: 3769
  year: 2010
  ident: ref_32
  article-title: Reactive oxygen species enhance TLR10 expression in the human monocytic cell line THP-1
  publication-title: Int. J. Mol. Sci.
  doi: 10.3390/ijms11103769
– volume: 38
  start-page: 1571
  year: 2006
  ident: ref_60
  article-title: Induction of interleukin-8 secretion and activation of ERK1/2, p38 MAPK signaling pathways by thrombin in dermal fibroblasts
  publication-title: Int. J. Biochem. Cell. Biol.
  doi: 10.1016/j.biocel.2006.03.016
– volume: 10
  start-page: 855
  year: 2004
  ident: ref_38
  article-title: The role of ROS and RNS in regulating life and death of blood monocytes
  publication-title: Curr. Pharm. Des.
  doi: 10.2174/1381612043452866
– volume: 2013
  start-page: 564961
  year: 2013
  ident: ref_21
  article-title: Oxidative stress in cardiovascular diseases and obesity: Role of p66Shc and protein kinase C
  publication-title: Oxid. Med. Cell. Longev.
– volume: 2
  start-page: 106
  year: 2002
  ident: ref_2
  article-title: Chemokine receptors: Multifaceted therapeutic targets
  publication-title: Nat. Rev. Immunol.
  doi: 10.1038/nri722
– ident: ref_55
  doi: 10.1101/533018
– volume: 82
  start-page: 1257
  year: 2007
  ident: ref_57
  article-title: Hypoxia causes an increase in phagocytosis by macrophages in a HIF-1alpha-dependent manner
  publication-title: J. Leukoc. Biol.
  doi: 10.1189/jlb.0307195
– volume: 1758
  start-page: 994
  year: 2006
  ident: ref_39
  article-title: Membrane transport of hydrogen peroxide
  publication-title: Biochim. Biophys. Acta
  doi: 10.1016/j.bbamem.2006.02.015
– volume: 16
  start-page: 469
  year: 2016
  ident: ref_53
  article-title: The unfolded protein response in immunity and inflammation
  publication-title: Nat. Rev. Immunol.
  doi: 10.1038/nri.2016.62
– volume: 25
  start-page: 677
  year: 2004
  ident: ref_3
  article-title: The chemokine system in diverse forms of macrophage activation and polarization
  publication-title: Trends Immunol.
  doi: 10.1016/j.it.2004.09.015
– volume: 62
  start-page: 303
  year: 2006
  ident: ref_19
  article-title: Mitochondrial oxidative stress and inflammation: An slalom to obesity and insulin resistance
  publication-title: J. Physiol. Biochem.
  doi: 10.1007/BF03165759
– volume: 94
  start-page: 534
  year: 2004
  ident: ref_20
  article-title: Interleukin-6 induces oxidative stress and endothelial dysfunction by overexpression of the angiotensin II type 1 receptor
  publication-title: Circ. Res.
  doi: 10.1161/01.RES.0000115557.25127.8D
– volume: 67
  start-page: 688
  year: 1999
  ident: ref_61
  article-title: Involvement of mitogen-activated protein kinase pathways in interleukin-8 production by human monocytes and polymorphonuclear cells stimulated with lipopolysaccharide or Mycoplasma fermentans membrane lipoproteins
  publication-title: Infect. Immun.
  doi: 10.1128/IAI.67.2.688-693.1999
– volume: 86
  start-page: 1267
  year: 2001
  ident: ref_33
  article-title: Regulation of interleukin 8 production and gene expression in human adipose tissue in vitro
  publication-title: J. Clin. Endocrinol Metab.
– volume: 35
  start-page: 40
  year: 2016
  ident: ref_47
  article-title: Induction of IL-8(CXCL8) and MCP-1(CCL2) with oxidative stress and its inhibition with N-acetyl cysteine (NAC) in cell culture model using HK-2 cell
  publication-title: Transplant. Immunol.
  doi: 10.1016/j.trim.2016.02.003
– volume: 20
  start-page: 87
  year: 2010
  ident: ref_13
  article-title: Tumor necrosis factor-alpha signaling in macrophages
  publication-title: Crit. Rev. Eukaryot. Gene Expr.
  doi: 10.1615/CritRevEukarGeneExpr.v20.i2.10
– volume: 17
  start-page: 1464
  year: 2007
  ident: ref_6
  article-title: Proinflammatory cytokines in obesity: Impact of type 2 diabetes mellitus and gastric bypass
  publication-title: Obes. Surg.
  doi: 10.1007/s11695-008-9424-z
– volume: 28
  start-page: 1456
  year: 2000
  ident: ref_35
  article-title: Reactive oxygen species, cell signaling, and cell injury
  publication-title: Free Radic. Biol. Med.
  doi: 10.1016/S0891-5849(00)00252-5
– volume: 23
  start-page: 26
  year: 2018
  ident: ref_58
  article-title: HIF-1alpha promotes the migration and invasion of hepatocellular carcinoma cells via the IL-8-NF-kappaB axis
  publication-title: Cell. Mol. Biol. Lett.
  doi: 10.1186/s11658-018-0077-1
– volume: 27
  start-page: 1091
  year: 1997
  ident: ref_67
  article-title: Cytokine induction of monocyte chemoattractant protein-1 gene expression in human endothelial cells depends on the cooperative action of NF-kappa B and AP-1
  publication-title: Eur. J. Immunol.
  doi: 10.1002/eji.1830270508
– volume: 59
  start-page: 505
  year: 2000
  ident: ref_68
  article-title: Obesity: Criteria and classification
  publication-title: Proc. Nutr. Soc.
  doi: 10.1017/S0029665100000732
– volume: 32
  start-page: 537
  year: 2000
  ident: ref_8
  article-title: Interleukin-8 production in human adipose tissue. inhibitory effects of anti-diabetic compounds, the thiazolidinedione ciglitazone and the biguanide metformin
  publication-title: Horm. Metab. Res.
  doi: 10.1055/s-2007-978682
– volume: 107
  start-page: 4675
  year: 2010
  ident: ref_25
  article-title: Negative feedback control of HIF-1 through REDD1-regulated ROS suppresses tumorigenesis
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0907705107
– volume: 34
  start-page: 895
  year: 2014
  ident: ref_43
  article-title: Neuroprotection induced by N-acetylcysteine and selenium against traumatic brain injury-induced apoptosis and calcium entry in hippocampus of rat
  publication-title: Cell. Mol. Neurobiol.
  doi: 10.1007/s10571-014-0069-2
– volume: 48
  start-page: 1263
  year: 1995
  ident: ref_66
  article-title: Activation of nuclear factor-kappa B correlates with MCP-1 expression by human mesangial cells
  publication-title: Kidney Int.
  doi: 10.1038/ki.1995.410
– volume: 30
  start-page: 1347
  year: 2006
  ident: ref_14
  article-title: Circulating levels of MCP-1 and IL-8 are elevated in human obese subjects and associated with obesity-related parameters
  publication-title: Int. J. Obesity.
  doi: 10.1038/sj.ijo.0803259
– volume: 50
  start-page: 101
  year: 1994
  ident: ref_11
  article-title: Regulation of monocyte chemoattractant protein-1 expression in adult human non-neoplastic astrocytes is sensitive to tumor necrosis factor (TNF) or antibody to the 55-kDa TNF receptor
  publication-title: J. Neuroimmunol.
  doi: 10.1016/0165-5728(94)90220-8
– volume: 389
  start-page: 610
  year: 1997
  ident: ref_29
  article-title: Protection from obesity-induced insulin resistance in mice lacking TNF-alpha function
  publication-title: Nature
  doi: 10.1038/39335
– volume: 62
  start-page: 51
  year: 2013
  ident: ref_46
  article-title: High dose N-acetyl cysteine improves inflammatory response and outcome in patients with COPD exacerbations
  publication-title: Egypt. J. Chest Dis. Tuberc.
  doi: 10.1016/j.ejcdt.2013.02.012
– volume: 139
  start-page: 788
  year: 1987
  ident: ref_9
  article-title: Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: Partial characterization and separation from interleukin 1 (IL 1)
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.139.3.788
– volume: 50
  start-page: 184
  year: 2000
  ident: ref_12
  article-title: TNF alpha and the TNF receptor superfamily: Structure-function relationship(s)
  publication-title: Microsc. Res. Tech.
  doi: 10.1002/1097-0029(20000801)50:3<184::AID-JEMT2>3.0.CO;2-H
– volume: 20
  start-page: 425
  year: 2016
  ident: ref_62
  article-title: Increased MCP-1 gene expression in monocytes of severe OSA patients and under intermittent hypoxia
  publication-title: Sleep Breath.
  doi: 10.1007/s11325-015-1252-5
– volume: 11
  start-page: 241
  year: 2009
  ident: ref_49
  article-title: Curcumin supplementation lowers TNF-alpha, IL-6, IL-8, and MCP-1 secretion in high glucose-treated cultured monocytes and blood levels of TNF-alpha, IL-6, MCP-1, glucose, and glycosylated hemoglobin in diabetic rats
  publication-title: Antioxid. Redox. Signal.
  doi: 10.1089/ars.2008.2140
– volume: 7
  start-page: 787
  year: 2001
  ident: ref_63
  article-title: Nuclear factor-kappa B is essential for up-regulation of interleukin-8 expression in human amnion and cervical epithelial cells
  publication-title: Mol. Hum. Reprod.
  doi: 10.1093/molehr/7.8.787
– volume: 289
  start-page: C582
  year: 2005
  ident: ref_52
  article-title: Bidirectional regulation of monocyte chemoattractant protein-1 gene at distinct sites of its promoter by nitric oxide in vascular smooth muscle cells
  publication-title: Am. J. Physiol. Cell Physiol.
  doi: 10.1152/ajpcell.00558.2004
– volume: 275
  start-page: 26765
  year: 2000
  ident: ref_56
  article-title: Nonhypoxic pathway mediates the induction of hypoxia-inducible factor 1alpha in vascular smooth muscle cells
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(19)61441-9
– volume: 276
  start-page: 42728
  year: 2001
  ident: ref_36
  article-title: Reactive oxygen species are downstream products of TRAF-mediated signal transduction
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M103074200
– volume: 4
  start-page: 12
  year: 2007
  ident: ref_59
  article-title: Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes
  publication-title: J. Neuroinflamm.
  doi: 10.1186/1742-2094-4-12
– volume: 135
  start-page: 127
  year: 2012
  ident: ref_15
  article-title: Association of TNF-alpha with insulin resistance in type 2 diabetes mellitus
  publication-title: Indian J. Med. Res.
  doi: 10.4103/0971-5916.93435
– volume: 38
  start-page: 176
  year: 2008
  ident: ref_51
  article-title: CHOP transcription factor mediates IL-8 signaling in cystic fibrosis bronchial epithelial cells
  publication-title: Am. J. Respir Cell. Mol. Biol.
  doi: 10.1165/rcmb.2007-0197OC
– volume: 87
  start-page: 4602
  year: 2002
  ident: ref_4
  article-title: Plasma interleukin-8 concentrations are increased in obese subjects and related to fat mass and tumor necrosis factor-alpha system
  publication-title: J. Clin. Endocrinol. Metab.
  doi: 10.1210/jc.2002-020135
– volume: 43
  start-page: 775
  year: 2018
  ident: ref_48
  article-title: Curcumin Inhibits Monocyte Chemoattractant Protein-1 Expression in TNF-α induced Astrocytes Through AMPK Pathway
  publication-title: Neurochem. Res.
  doi: 10.1007/s11064-018-2479-x
– volume: 2012
  start-page: 943706
  year: 2012
  ident: ref_27
  article-title: Inflammation and oxidative stress in obesity, metabolic syndrome, and diabetes
  publication-title: Exp. Diabetes Res.
  doi: 10.1155/2012/943706
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Snippet IL-8/MCP-1 act as neutrophil/monocyte chemoattractants, respectively. Oxidative stress emerges as a key player in the pathophysiology of obesity. However, it...
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proquest
crossref
SourceType Open Access Repository
Aggregation Database
Enrichment Source
Index Database
StartPage 10519
SubjectTerms Body mass index
Chemokines
Cytokines
Fibroblasts
Flow cytometry
Gene expression
Hypoxia
Inflammation
Insulin resistance
Kinases
Metabolic disorders
Metabolic syndrome
Microscopy
Neutrophils
Obesity
Overweight
Oxidative stress
Protein expression
Proteins
Reactive oxygen species
Tumor necrosis factor-TNF
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Title ROS/TNF-α Crosstalk Triggers the Expression of IL-8 and MCP-1 in Human Monocytic THP-1 Cells via the NF-κB and ERK1/2 Mediated Signaling
URI https://www.proquest.com/docview/2581003770
https://www.proquest.com/docview/2581828219
https://pubmed.ncbi.nlm.nih.gov/PMC8508672
Volume 22
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